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High-probability grants
According to our matching algorithm, Eric Johnson is the likely recipient of the following grants.
Years |
Recipients |
Code |
Title / Keywords |
Matching score |
2008 — 2009 |
Johnson, Eric A |
R21Activity Code Description: To encourage the development of new research activities in categorical program areas. (Support generally is restricted in level of support and in time.) |
Rapid and Flexible Snp Discovery and Genotyping
[unreadable] DESCRIPTION (provided by applicant): Individuals differ in the exact nucleotide sequence of their genomes. These differences can be utilized to track the inheritance of chromosomal regions, allowing mutations produced by genetic screens or disease-causing gene alleles to be rapidly mapped. Approaches for identifying these changes, called single nucleotide polymorphisms or SNPs are rapidly improving, and yet both the discovery and genotyping of SNPs in all organisms, but particularly ones without known genome sequences, remains a major undertaking. The long-term objectives of this application are to develop techniques for SNP discovery and genotyping that are inexpensive, high-throughput, and are applicable to a variety of organisms. As sequence information becomes inexpensive and custom high-density tiling microarrays become readily available, Restriction site Associated DNA (RAD) tags provide superior characteristics for genotyping. Thousands of RAD tag markers are assayable with a single hybridization, the polymorphism is converted into a simple binary presence or absence of a DNA fragment several hundred nucleotides long, and the marker allows the rapid genotyping of large populations on a marker by marker basis, speeding fine-scale mapping. The specific aims are to 1) Optimize protocols for the discovery of RAD markers on tiling arrays and characterizing the design parameters of RAD markers when genetic mapping with pooled populations. 2) Develop methods for the high-throughput isolation of RAD tags, which will allow the genotyping of many individuals in a population. The RAD marker approach allows inexpensive, high- throughput screening of high-density genetic markers in any organism, a significant benefit for researchers wishing to understand the genetic basis of phenotype.Project narrative [unreadable] [unreadable] Identifying the genetic changes that cause disease in humans, or affect a biological process in a model organism, has been difficult. This application develops methods to allow genetic changes to be rapidly and inexpensively mapped to a small region of the genome. [unreadable] [unreadable] [unreadable]
|
0.958 |
2011 — 2012 |
Johnson, Eric A |
R21Activity Code Description: To encourage the development of new research activities in categorical program areas. (Support generally is restricted in level of support and in time.) |
Conversion of Short-Read Sequence Data to Locally-Assembled Long Contigs
DESCRIPTION (provided by applicant): Next-generation sequencing has created of wealth of information about the genetic basis of disease and natural variation. The major flaw of next-generation sequencers is the short read length, which creates problems in piecing together a complete genome. The goal of this proposal is to use molecular biology to create DNA libraries that can be sequenced and more easily assembled into long contiguous stretches. The proposal will first focus on creating contig sequences of 5,000 nucleotides that have been assembled from each sub-region of the genome. Next, the goal is to create stretches of 50,000 nucleotide assemblies, also pieced together in parallel across the genome. Computer scripts to optimize the assembly of the genomic regions will also be developed. These longer contiguous sequences will greatly improve the ability of researchers to accurately determine the proper order of genes in a genome, which will speed discovery of the DNA changes that give rise to altered phenotypes in model organisms, non-model organisms and humans. PUBLIC HEALTH RELEVANCE: Understanding the sequence of a genome helps researchers make new discoveries about biology and health more quickly. This application proposes to develop methods to help put a genome sequence together in the proper order, simplifying the process of reading a genome.
|
0.958 |
2012 — 2016 |
Johnson, Eric A |
P50Activity Code Description: To support any part of the full range of research and development from very basic to clinical; may involve ancillary supportive activities such as protracted patient care necessary to the primary research or R&D effort. The spectrum of activities comprises a multidisciplinary attack on a specific disease entity or biomedical problem area. These grants differ from program project grants in that they are usually developed in response to an announcement of the programmatic needs of an Institute or Division and subsequently receive continuous attention from its staff. Centers may also serve as regional or national resources for special research purposes. |
Genomics Core
GENOMICS CORE AND HUB OF INNOVATION Mission Research Areas I and III will use massively-parallel sequencing to assay the richness of microbial communities and the response of fish hosts to those communities. The Genomics Core Facility will assist in library preparation and routine analysis of raw data to allow Center members to perform genomic assays quickly and with the highest possible quality.
|
0.958 |