2010 — 2012 |
Evans, Jennifer Anne |
F32Activity Code Description: To provide postdoctoral research training to individuals to broaden their scientific background and extend their potential for research in specified health-related areas. |
Investigating Circadian Communication Within the Brain and Body @ Morehouse School of Medicine
DESCRIPTION (provided by applicant): How oscillators within the SCN interact with one another and the rest of the brain remains an ill-defined but critical area of research. Different regions of the SCN project to largely overlapping brain regions;however, it is unknown whether output pathways from each region convey redundant or distinct signals. To assess regional interactions and the functional contribution of different SCN regions, I have dissociated rhythms in the dorsal and ventral SCN using an in vivo photoperiodic manipulation. To track the phase of individual neurons within the SCN, we employ real-time bioluminescence imaging of SCN slices from the PER2:LUC mouse, a transgenic mouse model where a PER2 production within individual cells is monitored using a firefly luciferase reporter. In preliminary studies, I have used an ultra long photoperiod (20h light: 4h darkness, LD 20:4) to reorganize the SCN into dorsal and ventral regions that express dissociated rhythms on the first cycle in vitro. Over the subsequent cycles in vitro, the phase difference between dorsal and ventral SCN is reduced, suggesting that dissociated regions interact in vitro. I propose to test the hypotheses 1) that changes in phase relationships over time in vitro depend on coupling between SCN regions and 2) that dorsal and ventral SCN convey functionally distinct timing signals to downstream tissues. To investigate these questions, I will use real-time bioluminescence imaging of multiple tissue types, advanced computational analyses of the imaging data, pharmacological manipulations, and immunohistochemistry for clock gene expression in SCN targets. My long-term objective is to understand how neural oscillators within the SCN interact to form a functional pacemaker capable of regulating rhythms in behavior and physiology. PUBLIC HEALTH RELEVANCE: Circadian organization has long been one of the strongest model systems for investigating the links between brain function and complex behavior, with research providing insight into multiple levels of analysis. At the systems level, the SCN was among the first discrete brain regions to be conclusively linked to the regulation of complex behavior and continues to provide an outstanding model for investigating the fundamental principles that govern brain systems and neural function in mammals. Cellular and molecular studies of circadian function are revealing new links between the circadian system, sleep, metabolic disorder, cancer, cardiovascular disease, affective disorders, and other health dysfunctions. Such developments signal the import of circadian biology for human health and pathology. Herein I propose to use a photoperiodic manipulation to investigate the principles of how SCN oscillators interact with each other and the rest of the body. In humans and rodents, changes in photoperiod are associated with changes in a variety of behavioral and physiological systems, including reproductive function, immune function, metabolic function, cognitive function, and affective behavior. Since the present studies investigate the consequences of photoperiodic manipulations for circadian organization, the results are highly pertinent to human health problems associated with changes in the duration of daily bright light exposure, such as that experienced during changing seasons and night shift work. Moreover, by investigating the fundamental principles of circadian organization, the studies proposed here may lead to novel diagnostic and therapeutic approaches for addressing other pathological states related to circadian clock dysfunction.
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2015 — 2019 |
Evans, Jennifer Anne |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Inhibitory Feedback Mechanisms That Couple Circadian Clock Neurons in Mammals
? DESCRIPTION (provided by applicant): Neuron ensembles display coordinated activity patterns that arise from feedback connections among the constituent neurons; however, the mechanisms that synchronize neural activity in time and space remain poorly understood. This is a fundamental gap in our understanding because neuronal synchronization is critical for normal brain function and its dysfunction is implicated in numerous neurological disorders (e.g., epilepsy, Parkinson's disease, schizophrenia). An excellent model system for studying the principles and mechanisms underlying neuronal synchronization is the suprachiasmatic nucleus (SCN). The SCN is a network of neuronal oscillators that programs circadian rhythms of behavior and physiology in mammals to ensure biological events occur at the appropriate time of day. As a population, SCN neurons display coordinated activity patterns that are integral to circadian clock function; however, the signaling mechanisms that regulate this process remain ill defined. One major obstacle barring progress on this front is the challenge of investigating neuronal interactions while the SCN network is in a functional state. To address this important issue, we have developed a novel analytical assay that quantifies the dynamic process by which SCN neurons interact using an ex vivo slice preparation. In the proposed studies, we will employ this analytical assay together with specific pharmacological tools and innovative genetic manipulations to investigate how different subclasses of SCN neurons interact to control circadian behavior. First, we will test the hypothesis that feedback within the SCN network is necessary for neuronal synchronization in vitro. Second, we will define the intracellular signaling mechanisms that mediate this feedback. Last, we will evaluate whether feedback in the SCN network is required for circadian rhythms in vivo. By defining the functional role of novel signaling pathways that modulate SCN function in vitro and in vivo, our work will significantly advance understanding of the circuit mechanisms that bind SCN neurons into a synchronized network. Greater insight into SCN circuitry is expected to help develop novel therapies to prevent and alleviate the adverse health consequences of disrupted clock function, such as sleep disorders.
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