Area:
Genetics, Neuroscience Biology, Ophthalmology
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High-probability grants
According to our matching algorithm, Joseph A. Brzezinski is the likely recipient of the following grants.
Years |
Recipients |
Code |
Title / Keywords |
Matching score |
2008 — 2009 |
Brzezinski, Joseph Allan |
F32Activity Code Description: To provide postdoctoral research training to individuals to broaden their scientific background and extend their potential for research in specified health-related areas. |
Visual Regeneration by Cone Photoreceptor Replacement @ University of Washington
[unreadable] DESCRIPTION (provided by applicant): Photoreceptor degenerations lead to visual impairment and blindness. Since human retinas are incapable of regeneration, cell replacement therapy is an attractive approach to restore lost photoreceptors. Of particular interest is programming human embryonic stem (hES) cells into cone photoreceptors that can be used to restore high acuity daytime (photopic) vision in diseased retinas after transplant. To implement this strategy requires knowledge of the factors that control cone development, the conditions permissive for cone transplantation into adult retinas, and whether hES cell derived cones can restore photopic vision in diseased retinas. We will address these problems in the following specific aims: 1. To determine how cell extrinsic factors control cone photoreceptor specification and differentiation. Cell non-autonomous effects can control the numbers of each cell type in the retina; but how do they regulate cones? To test this, we will treat dissociated retinal progenitors with 6 candidate factors. To specifically label cones, retinal progenitors from M-opsin:GFP and thyroid hormone 02 knock-in (TR[52) mouse retinas will be collected. Dissociated cells will be labeled with BrdU, and treated with candidate factors for one week. The effects of these extrinsic factors on cone fate and differentiation will be quantified. 2. To determine the donor cell conditions that promote efficient transplantation of functional cones in adult retinas. Previous data suggests postmitotic, undifferentiated cells integrate best into adult retinas. To test this for cones, we will purify cones from TRB2 knock-in mice by flow cytometry and subretinally transplant them into wild-type mice. After 2 weeks, retinas will be scored for transplanted cones. The extent of integration at each time-point will be compared to determine what stages are permissive for cone transplantation. 3. To restore photopic vision in diseased retinas by transplantation of cones derived from hES cells. Our laboratory has developed a protocol to convert hES cells into retinal progenitor cells. To test whether these cells can restore photopic vision we will treat hES cells with extrinsic factors to enrich for cones. Next, we will transplant cone enriched hES cells into adult Crx null mice, which have no functional photoreceptors. We will measure photopic vision restoration 2-6 weeks later by electrophysiology and histology. [unreadable] [unreadable]
|
0.955 |
2014 — 2021 |
Brzezinski, Joseph A |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Mechanisms of Cell Fate Specification and Competence Regulation in Photoreceptors @ University of Colorado Denver
DESCRIPTION (provided by applicant): About 10 million Americans suffer from age-related macular degeneration (AMD) or diabetic retinopathy, and prevalence is expected to increase 50-75% by 2030. These diseases are associated with rod and/or cone photoreceptor cell death, which causes permanent vision loss. Current therapies can limit photoreceptor loss, but do not restore vision to blind patients. To overcome this, researchers have attempted to generate new photoreceptors from stem cells and transplant them into diseased retinas. While promising, this approach remains too inefficient and time-consuming to be clinically feasible. This is because the earliest events in photoreceptor development- competence (cell fate potential) and specification (cell fate commitment) - are poorly understood. The project objective is to understand these early events in mice; an excellent model system for human retinal development. The first aim of this project is to determine how a key early photoreceptor gene, Blimp1, is regulated during development. This will be done by investigating the requisite sequence from a recently discovered Blimp1 enhancer with high-throughput organotypic culture assays. Using a proteomics approach, the transcriptional regulators that bind to the Blimp1 enhancer will be discovered. These factors will then be investigated for their effect on Blimp1 expression and photoreceptor fate specification. The second aim of this project is to characterize how photoreceptor competence is regulated during development. The temporal aspects of photoreceptor competence will be investigated by narrowly altering competence in specific cell types, for different lengths of time, and at different stages of development. This project will uncover the mechanisms that regulate early events in photoreceptor development; information that is critical for the successful design and implementation of photoreceptor cell replacement therapies. These therapies have tremendous potential to reverse blindness in millions of people world-wide.
|
0.943 |