1988 — 1992 |
Haro, Luis S |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Structural Studies of Receptors For Lactogenic Hormones @ Whittier Institute For Diabetes &Endoc
During pregnancy, the lactogenic hormones prolactin and placental lactogen participate in the regulation of processes such as mammary gland differentiation, steroidogenesis, somatic growth and intermediary metabolism in a variety of animals. Regulation of these processes is achieved through fluctuations in the levels of the lactogenic hormones. We know that lactogenic hormones are structurally similar and possess overlapping biological activities, but very little is known about their receptors. It is not known whether these hormones send a biochemical signal through a receptor unique to each hormone or, alternatively, whether they function through a common receptor. Since the lactogenic hormones influence a variety of biological processes, it is important to have knowledge about receptors for each specific lactogen over the course of pregnancy. The mouse will be used as a biological model to study lactogenic receptors over the course of gestation. A prolactin receptor will be isolated from mouse mammary glands. The purified receptor will be structurally analyzed by peptide mapping, amino acid analysis, N-terminal analysis, and other biochemical means. An antiserum will be generated with purified prolactin receptor and employed as an immunochemical probe. After crosslinking radiolabelled lactogens to receptors from organs removed on different days of gestation, hormone- receptor complexes will be analyzed by two-dimensional polyacrylamide gel electrophoresis, immunoblotting and autoradiography for determination of the multiplicity of lactogenic receptors. Electroimmunochemical techniques will be used to ascertain structural similarities that exist among prolactin receptors of different target organs. Ontogeny of lactogenic receptors during pregnancy in both the mother and fetus will be monitored by electrophoresis and immunostaining of receptors from several tissues. The potency and specificity of the interaction of glycosylated prolactin with receptors will be examined using radioreceptorassays, crosslinking and immunochemical methodologies. These studies should provide information that will improve our understanding about receptors for lactogenic hormones in the mouse, and perhaps will lead to an understanding about receptors for human lactogenic hormones.
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0.91 |
1989 — 1990 |
Haro, Luis S |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Receptors For Lactogenic Hormones @ Whittier Institute For Diabetes &Endoc
During pregnancy, the lactogenic hormones prolactin and placental lactogen participate in the regulation of processes such as mammary gland differentiation, steroidogenesis, somatic growth and intermediary metabolism in a variety of animals. Regulation of these processes is achieved through fluctuations in the levels of the lactogenic hormones. We know that lactogenic hormones are structurally similar and possess overlapping biological activities, but very little is known about their receptors. It is not known whether these hormones send a biochemical signal through a receptor unique to each hormone or, alternatively, whether they function through a common receptor. Since the lactogenic hormones influence a variety of biological processes, it is important to have knowledge about receptors for each specific lactogen over the course of pregnancy. The mouse will be used as a biological model to study lactogenic receptors over the course of gestation. A prolactin receptor will be isolated from mouse mammary glands. The purified receptor will be structurally analyzed by peptide mapping, amino acid analysis, N-terminal analysis, and other biochemical means. An antiserum will be generated with purified prolactin receptor and employed as an immunochemical probe. After crosslinking radiolabelled lactogens to receptors from organs removed on different days of gestation, hormone- receptor complexes will be analyzed by two-dimensional polyacrylamide gel electrophoresis, immunoblotting and autoradiography for determination of the multiplicity of lactogenic receptors. Electroimmunochemical techniques will be used to ascertain structural similarities that exist among prolactin receptors of different target organs. Ontogeny of lactogenic receptors during pregnancy in both the mother and fetus will be monitored by electrophoresis and immunostaining of receptors from several tissues. The potency and specificity of the interaction of glycosylated prolactin with receptors will be examined using radioreceptorassays, crosslinking and immunochemical methodologies. These studies should provide information that will improve our understanding about receptors for lactogenic hormones in the mouse, and perhaps will lead to an understanding about receptors for human lactogenic hormones.
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0.91 |
1993 — 1995 |
Haro, Luis S |
S03Activity Code Description: Undocumented code - click on the grant title for more information. |
Minority High School Student Research Apprentice Program @ University of Texas San Antonio
minority institution research support; secondary schools;
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1 |
1995 — 2002 |
Haro, Luis S |
S06Activity Code Description: To strengthen the biomedical research and research training capability of ethnic minority institutions, and thus establish a more favorable milieu for increasing the involvement of minority faculty and students in biomedical research. |
Biological and Biochemical Properties of Glycosylated Human Growth Hormone @ University of Texas San Antonio
protein isoforms; somatotropin; chemical structure function; glycosylation; protein sequence; oligosaccharides; cell cycle; lipid metabolism; carbohydrate structure; lymphatic tissue; glucose metabolism; gel electrophoresis; monoclonal antibody; enzyme linked immunosorbent assay; laboratory mouse; laboratory rat; high performance liquid chromatography; radioimmunoassay; western blottings; gel filtration chromatography;
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1 |
2003 — 2004 |
Haro, Luis S |
T36Activity Code Description: Undocumented code - click on the grant title for more information. |
Sacnas: Initiatives For Equity in Science @ Society For the Adv Chicanos/Native Amer
DESCRIPTION (provided by applicant): SACNAS requests funding from the National Institutes of General Medical Sciences Minority Access to Research Careers program, for underrepresented undergraduate and graduate students, and professionals to encourage and develop an interest in biomedical sciences. Humans are a natural resource that continue to be underdeveloped in the U.S. and within the U.S. scientific community. This is particularly true for people who are members of minority groups, especially Chicanos and Native Americans. A recent NSF study (NSF 00 327) shows that while minorities make up nearly 25% of population in the U.S., Chicanos, Latinos, Native American and African Americans make up less than 6% of all Ph.D.s in science, engineering and mathematics (SEM). SEM are areas of expertise that are the comerstones for the future of this country. The underrepresentation of these people in the scientific enterprise is a serious national problem with many contributing factors (Chronicle of Higher Ed, Jan 2002). The Society for Advancement of Chicanos/Latinos and Native Americans (SACNAS) seeks to change this statistic by encouraging all underrepresented groups - while focusing on Chicanos/Latinos and Native Americans- to pursue advanced degrees in science, mathematics and engineering. SACNAS' mission and that of the NIH MARC program are congruent. The proposed activities integrate biomedical research and promote educational opportunities in NIH relevant areas. We have solicited funds from other sources to support scientists and budding scientists with interests in other areas of research. The hypothesis to be tested is that, with the active mentoring, career development, and specific interventions provided by SACNAS as supported by NIH, undergraduate, graduate, postdoctoral researchers, and professional minority scientists will be retained in science and will achieve excellence in this endeavor. SACNAS has a 15-year history of annual conferences with workshops, scientific sessions, student presentations, and mentoring activities. Past and current SACNAS activities are a foundation, and the springboard we shall use to launch the following specific aims: Aim 1. Provide an annual conference that enhances career development and higher education of undergraduate students (285), pre- (34) and post- (20) doctoral researchers (20) and faculty (5) interested in biomedical sciences; Aim 2. Provide year-round activities that will enhance student, postdoctoral and faculty development; and Aim 3. Evaluate the effectiveness of the proposed activities. The rationale for the proposed activities in this application is that without early, specific and well thought-out interventions, and without opportunities for students, postdoctoral researchers and faculty to take part in career and educational development our target communities will remain underrepresented in scientific endeavors.
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0.927 |
2004 |
Haro, Luis S |
S06Activity Code Description: To strengthen the biomedical research and research training capability of ethnic minority institutions, and thus establish a more favorable milieu for increasing the involvement of minority faculty and students in biomedical research. |
Protein Separation by Weak Base-Conjugate Acid Gradient Ph Chromatofocusing-Pilot @ University of Texas San Antonio |
1 |