Markus Hardt, Ph.D.

Affiliations: 
2002 Louisiana State University, Baton Rouge, LA, United States 
Area:
Biochemistry, Molecular Biology
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"Markus Hardt"
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Roger A. Laine grad student 2002 Louisiana State
 (Study of a Bacillus circulans chitin -binding domain by a green fluorescent protein binding assay and detection of lysozymes by improved zymograms.)
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Publications

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Niles R, Witkowska HE, Allen S, et al. (2009) Acid-catalyzed oxygen-18 labeling of peptides. Analytical Chemistry. 81: 2804-9
Denny P, Hagen FK, Hardt M, et al. (2008) The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions. Journal of Proteome Research. 7: 1994-2006
Robinson S, Niles RK, Witkowska HE, et al. (2008) A mass spectrometry-based strategy for detecting and characterizing endogenous proteinase activities in complex biological samples. Proteomics. 8: 435-45
Hardt M, Witkowska HE, Webb S, et al. (2005) Assessing the effects of diurnal variation on the composition of human parotid saliva: quantitative analysis of native peptides using iTRAQ reagents. Analytical Chemistry. 77: 4947-54
Hardt M, Thomas LR, Dixon SE, et al. (2005) Toward defining the human parotid gland salivary proteome and peptidome: identification and characterization using 2D SDS-PAGE, ultrafiltration, HPLC, and mass spectrometry. Biochemistry. 44: 2885-99
Hardt M, Laine RA. (2004) Mutation of active site residues in the chitin-binding domain ChBDChiA1 from chitinase A1 of Bacillus circulans alters substrate specificity: use of a green fluorescent protein binding assay. Archives of Biochemistry and Biophysics. 426: 286-97
Hardt M, Guo Y, Henderson G, et al. (2003) Zymogram with Remazol brilliant blue-labeled Micrococcus lysodeikticus cells for the detection of lysozymes: example of a new lysozyme activity in Formosan termite defense secretions. Analytical Biochemistry. 312: 73-6
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