Nancy L. Allbritton - Publications

Affiliations: 
Chemistry University of North Carolina, Chapel Hill, Chapel Hill, NC 
Area:
Signaling in Single Cells, Microfabricated Systems for Cellular Analysis
Website:
http://www.chem.unc.edu/people/faculty/allbritton/index.html

165 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2022 Gallion LA, Wang Y, Massaro A, Yao M, Petersen BV, Zhang Q, Huang W, Carr AJ, Zhang Q, Allbritton NL. "Fix and Click" for Assay of Sphingolipid Signaling in Single Primary Human Intestinal Epithelial Cells. Analytical Chemistry. PMID 35020354 DOI: 10.1021/acs.analchem.1c03503  0.301
2020 Hinman SS, Wang Y, Kim R, Allbritton NL. In vitro generation of self-renewing human intestinal epithelia over planar and shaped collagen hydrogels. Nature Protocols. PMID 33299154 DOI: 10.1038/s41596-020-00419-8  0.315
2020 LaBelle CA, Massaro A, Cortés-Llanos B, Sims CE, Allbritton NL. Image-Based Live Cell Sorting. Trends in Biotechnology. PMID 33190968 DOI: 10.1016/j.tibtech.2020.10.006  0.303
2020 Wang Y, Sims CE, Allbritton NL. Enterochromaffin Cell-Enriched Monolayer Platform for Assaying Serotonin Release from Human Primary Intestinal Cells. Analytical Chemistry. PMID 32819098 DOI: 10.1021/Acs.Analchem.0C02016  0.395
2020 Gallion LA, Anttila MM, Abraham DH, Proctor A, Allbritton NL. Preserving Single Cells in Space and Time for Analytical Assays. Trends in Analytical Chemistry : Trac. 122. PMID 32153309 DOI: 10.1016/J.Trac.2019.115723  0.629
2020 Nowotarski HL, Attayek PJ, Allbritton NL. Automated platform for cell selection and separation based on four-dimensional motility and matrix degradation. The Analyst. PMID 32083265 DOI: 10.1039/C9An02224D  0.43
2020 Smiddy NM, DiSalvo M, Allbritton-King JD, Allbritton NL. Microraft array-based platform for sorting of viable microcolonies based on cell-lethal immunoassay of intracellular proteins in microcolony biopsies. The Analyst. PMID 32048684 DOI: 10.1039/D0An00030B  0.448
2019 LaBelle C, Zhang R, Armistead P, Allbritton N. Assay and Isolation of Single Proliferating CD4+ Lymphocytes Using an Automated Microraft Array Platform. Ieee Transactions On Bio-Medical Engineering. PMID 31794384 DOI: 10.1109/Tbme.2019.2956081  0.338
2019 Speer JE, Wang Y, Fallon JK, Smith PC, Allbritton NL. Evaluation of human primary intestinal monolayers for drug metabolizing capabilities. Journal of Biological Engineering. 13: 82. PMID 31709009 DOI: 10.1186/S13036-019-0212-1  0.313
2019 Hinman SS, Wang Y, Allbritton NL. Photopatterned Membranes and Chemical Gradients Enable Scalable Phenotypic Organization of Primary Human Colon Epithelial Models. Analytical Chemistry. PMID 31692334 DOI: 10.1021/Acs.Analchem.9B04217  0.42
2019 Abraham DH, Anttila MM, Gallion LA, Petersen BV, Proctor A, Allbritton NL. Design of an automated capillary electrophoresis platform for single-cell analysis. Methods in Enzymology. 628: 191-221. PMID 31668230 DOI: 10.1016/Bs.Mie.2019.06.016  0.648
2019 Kim R, Attayek P, Wang Y, Furtado K, Tamayo R, Sims CE, Allbritton N. An in vitro intestinal platform with a self-sustaining oxygen gradient to study the human gut/microbiome interface. Biofabrication. PMID 31519008 DOI: 10.1088/1758-5090/Ab446E  0.345
2019 DiSalvo M, Smiddy NM, Allbritton NL. Automated sensing and splitting of stem cell colonies on microraft arrays. Apl Bioengineering. 3: 036106. PMID 31489396 DOI: 10.1063/1.5113719  0.331
2019 Proctor A, Wang Q, Lawrence DS, Allbritton NL. Selection and optimization of enzyme reporters for chemical cytometry. Methods in Enzymology. 622: 221-248. PMID 31155054 DOI: 10.1016/Bs.Mie.2019.02.023  0.63
2019 Speer JE, Gunasekara DB, Wang Y, Fallon JK, Attayek PJ, Smith PC, Sims CE, Allbritton NL. Molecular transport through primary human small intestinal monolayers by culture on a collagen scaffold with a gradient of chemical cross-linking. Journal of Biological Engineering. 13: 36. PMID 31061676 DOI: 10.1186/S13036-019-0165-4  0.753
2018 Dutton JS, Hinman SS, Kim R, Wang Y, Allbritton NL. Primary Cell-Derived Intestinal Models: Recapitulating Physiology. Trends in Biotechnology. PMID 30591184 DOI: 10.1016/J.Tibtech.2018.12.001  0.371
2018 Gunasekara D, Speer J, Wang Y, Nguyen DL, Reed MI, Smiddy NM, Parker JS, Fallon JK, Smith PC, Sims CE, Magness ST, Allbritton NL. A monolayer of primary colonic epithelium generated on a scaffold with a gradient of stiffness for drug transport studies. Analytical Chemistry. PMID 30350627 DOI: 10.1021/Acs.Analchem.8B02845  0.742
2018 Proctor A, Allbritton NL. "Fix and assay": separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative. The Analyst. PMID 30207332 DOI: 10.1039/C8An01353E  0.641
2018 Wang Y, Kim R, Hwang SJ, Dutton J, Sims CE, Allbritton NL. Analysis of Interleukin 8 Secretion by a Stem-Cell-Derived Human Intestinal Epithelial Monolayer Platform. Analytical Chemistry. PMID 30199234 DOI: 10.1021/Acs.Analchem.8B02835  0.313
2018 Williamson IA, Arnold JW, Samsa LA, Gaynor L, DiSalvo M, Cocchiaro JL, Carroll I, Azcarate-Peril MA, Rawls JF, Allbritton NL, Magness ST. A High-Throughput Organoid Microinjection Platform to Study Gastrointestinal Microbiota and Luminal Physiology. Cellular and Molecular Gastroenterology and Hepatology. 6: 301-319. PMID 30123820 DOI: 10.1016/J.Jcmgh.2018.05.004  0.302
2018 Kim R, Wang Y, Hwang SJ, Attayek PJ, Smiddy NM, Reed MI, Sims CE, Allbritton NL. Formation of arrays of planar, murine, intestinal crypts possessing a stem/proliferative cell compartment and differentiated cell zone. Lab On a Chip. PMID 29944153 DOI: 10.1039/C8Lc00332G  0.467
2018 Wang Y, Kim R, Gunasekara DB, Reed MI, DiSalvo M, Nguyen DL, Bultman SJ, Sims CE, Magness ST, Allbritton NL. Formation of Human Colonic Crypt Array by Application of Chemical Gradients Across a Shaped Epithelial Monolayer. Cellular and Molecular Gastroenterology and Hepatology. 5: 113-130. PMID 29693040 DOI: 10.1016/J.Jcmgh.2017.10.007  0.755
2018 Wang Y, Kim R, Hinman SS, Zwarycz B, Magness ST, Allbritton NL. Bioengineered Systems and Designer Matrices That Recapitulate the Intestinal Stem Cell Niche. Cellular and Molecular Gastroenterology and Hepatology. 5: 440-453.e1. PMID 29675459 DOI: 10.1016/J.Jcmgh.2018.01.008  0.434
2018 DiSalvo M, Harris D, Kantesaria S, Pena AN, Allbritton-King JD, Cole JH, Allbritton NL. Characterization of Tensioned PDMS Membranes for Imaging Cytometry on Microraft Arrays. Analytical Chemistry. PMID 29510027 DOI: 10.1021/Acs.Analchem.8B00176  0.333
2018 Vickerman BM, Anttila MM, Peterson BV, Allbritton NL, Lawrence DS. Design and Application of Sensors for Chemical Cytometry. Acs Chemical Biology. PMID 29376326 DOI: 10.1021/Acschembio.7B01009  0.434
2017 Wang Y, Gunasekara DB, Attayek PJ, Reed MI, DiSalvo M, Nguyen DL, Dutton JS, Lebhar MS, Bultman SJ, Sims CE, Magness ST, Allbritton NL. Generation of Mouse Colon Crypts. Acs Biomaterials Science & Engineering. 3: 2502-2513. PMID 30854421 DOI: 10.1021/Acsbiomaterials.7B00368  0.769
2017 Gunasekara DB, Disalvo M, Wang Y, Nguyen D, Reed MI, Speer J, Sims CE, Magness ST, Allbritton NL. Development of arrayed colonic organoids for screening of secretagogues associated with enterotoxins. Analytical Chemistry. PMID 29281259 DOI: 10.1021/Acs.Analchem.7B04032  0.766
2017 Fayer EL, Gilliland WM, Ramsey JM, Allbritton NL, Waters ML. N-Gemini peptides: cytosolic protease resistance via N-terminal dimerization of unstructured peptides. Chemical Communications (Cambridge, England). PMID 29230440 DOI: 10.1039/C7Cc06819K  0.316
2017 Wang Y, DiSalvo M, Gunasekara DB, Dutton J, Proctor A, Lebhar MS, Williamson IA, Speer J, Howard RL, Smiddy NM, Bultman SJ, Sims CE, Magness ST, Allbritton NL. Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells. Cellular and Molecular Gastroenterology and Hepatology. 4: 165-182.e7. PMID 29204504 DOI: 10.1016/J.Jcmgh.2017.02.011  0.785
2017 Bhatt AP, Gunasekara D, Speer JE, Reed MI, Peña A, Midkiff B, Magness S, Bultman S, Allbritton NL, Redinbo MR. NSAID-Induced Leaky Gut Modeled Using Polarized Monolayers of Primary Human Intestinal Epithelial Cells. Acs Infectious Diseases. PMID 29094594 DOI: 10.1021/Acsinfecdis.7B00139  0.736
2017 Waybright J, Huang W, Proctor A, Wang X, Allbritton NL, Zhang Q. Required hydrophobicity of fluorescent reporters for phosphatidylinositol family of lipid enzymes. Analytical and Bioanalytical Chemistry. PMID 28932942 DOI: 10.1007/S00216-017-0633-Y  0.545
2017 Proctor A, Sims CE, Allbritton NL. Chemical fixation to arrest phospholipid signaling for chemical cytometry. Journal of Chromatography. A. PMID 28528682 DOI: 10.1016/J.Chroma.2017.05.022  0.646
2017 Wang Y, Gunasekara DB, Reed MI, DiSalvo M, Bultman SJ, Sims CE, Magness ST, Allbritton NL. A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium. Biomaterials. 128: 44-55. PMID 28288348 DOI: 10.1016/J.Biomaterials.2017.03.005  0.773
2016 Attayek PJ, Waugh JP, Hunsucker SA, Grayeski PJ, Sims CE, Armistead PM, Allbritton NL. Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9. Biosensors & Bioelectronics. 91: 175-182. PMID 28006686 DOI: 10.1016/J.Bios.2016.12.019  0.39
2016 Attayek PJ, Hunsucker SA, Sims CE, Allbritton NL, Armistead PM. Identification and isolation of antigen-specific cytotoxic T lymphocytes with an automated microraft sorting system. Integrative Biology : Quantitative Biosciences From Nano to Macro. PMID 27853786 DOI: 10.1039/C6Ib00168H  0.38
2016 Proctor A, Zigoneanu IG, Wang Q, Sims CE, Lawrence DS, Allbritton NL. Development of a protease-resistant reporter to quantify BCR-ABL activity in intact cells. The Analyst. PMID 27704073 DOI: 10.1039/C6An01378C  0.596
2016 Mainz ER, Wang Q, Lawrence DS, Allbritton NL. An Integrated Chemical Cytometry Method: Shining a Light on Akt Activity in Single Cells. Angewandte Chemie (International Ed. in English). PMID 27647713 DOI: 10.1002/Anie.201606914  0.419
2016 Welch JD, Williams LA, DiSalvo M, Brandt AT, Marayati R, Sims CE, Allbritton NL, Prins JF, Yeh JJ, Jones CD. Selective single cell isolation for genomics using microraft arrays. Nucleic Acids Research. PMID 27530426 DOI: 10.1093/Nar/Gkw700  0.429
2016 Attayek PJ, Ahmad AA, Wang Y, Williamson I, Sims CE, Magness ST, Allbritton NL. In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment. Plos One. 11: e0153795. PMID 27100890 DOI: 10.1371/Journal.Pone.0153795  0.329
2016 Koh J, Allbritton NL, Sosa JA. Single-cell approaches for molecular classification of endocrine tumors. Current Opinion in Oncology. 28: 43-9. PMID 26632769 DOI: 10.1097/Cco.0000000000000246  0.367
2015 Turner AH, Lebhar MS, Proctor A, Wang Q, Lawrence DS, Allbritton NL. RATIONAL DESIGN OF A DEPHOSPHORYLATION-RESISTANT REPORTER ENABLES SINGLE-CELL MEASUREMENT OF TYROSINE KINASE ACTIVITY. Acs Chemical Biology. PMID 26587880 DOI: 10.1021/Acschembio.5B00667  0.586
2015 Attayek PJ, Hunsucker SA, Wang Y, Sims CE, Armistead PM, Allbritton NL. An array-based platform to select, release and capture EBV-infected cells based on intercellular adhesion. Analytical Chemistry. PMID 26558605 DOI: 10.1021/Acs.Analchem.5B03579  0.447
2015 Melvin AT, Dumberger LD, Woss GS, Waters ML, Allbritton NL. Identification of a p53-based portable degron based on the MDM2-p53 binding region. The Analyst. PMID 26456660 DOI: 10.1039/C5An01429H  0.317
2015 Ornoff DM, Wang Y, Proctor A, Shah AS, Allbritton NL. Co-fabrication of chitosan and epoxy photoresist to form microwell arrays with permeable hydrogel bottoms. Biomaterials. 74: 77-88. PMID 26447557 DOI: 10.1016/J.Biomaterials.2015.09.032  0.621
2015 Zigoneanu IG, Sims CE, Allbritton NL. Separation of peptide fragments of a protein kinase C substrate fused to a β-hairpin by capillary electrophoresis. Analytical and Bioanalytical Chemistry. PMID 26427499 DOI: 10.1007/S00216-015-9065-8  0.369
2015 Mainz ER, Dobes NC, Allbritton NL. Pronase E-Based Generation of Fluorescent Peptide Fragments: Tracking Intracellular Peptide Fate in Single Cells. Analytical Chemistry. 87: 7987-95. PMID 26171808 DOI: 10.1021/Acs.Analchem.5B01929  0.769
2015 Dickinson AJ, Meyer M, Pawlak EA, Gomez S, Jaspers I, Allbritton NL. Analysis of sphingosine kinase activity in single natural killer cells from peripheral blood. Integrative Biology : Quantitative Biosciences From Nano to Macro. 7: 392-401. PMID 25786072 DOI: 10.1039/C5Ib00007F  0.354
2015 Gracz AD, Williamson IA, Roche KC, Johnston MJ, Wang F, Wang Y, Attayek PJ, Balowski J, Liu XF, Laurenza RJ, Gaynor LT, Sims CE, Galanko JA, Li L, Allbritton NL, et al. A high-throughput platform for stem cell niche co-cultures and downstream gene expression analysis. Nature Cell Biology. 17: 340-9. PMID 25664616 DOI: 10.1038/Ncb3104  0.411
2015 Ahmad AA, Wang Y, Sims CE, Magness ST, Allbritton NL. Optimizing Wnt-3a and R-spondin1 concentrations for stem cell renewal and differentiation in intestinal organoids using a gradient-forming microdevice Rsc Advances. 5: 74881-74891. DOI: 10.1039/C5Ra14923A  0.348
2014 Dickinson AJ, Hunsucker SA, Armistead PM, Allbritton NL. Single-cell sphingosine kinase activity measurements in primary leukemia. Analytical and Bioanalytical Chemistry. 406: 7027-36. PMID 24980601 DOI: 10.1007/S00216-014-7974-6  0.388
2014 Shah PK, Herrera-Loeza SG, Sims CE, Yeh JJ, Allbritton NL. Small sample sorting of primary adherent cells by automated micropallet imaging and release. Cytometry. Part a : the Journal of the International Society For Analytical Cytology. 85: 642-9. PMID 24939722 DOI: 10.1002/Cyto.A.22480  0.349
2014 Shah PK, Walker MP, Sims CE, Major MB, Allbritton NL. Dynamics and evolution of β-catenin-dependent Wnt signaling revealed through massively parallel clonogenic screening. Integrative Biology : Quantitative Biosciences From Nano to Macro. 6: 673-84. PMID 24871928 DOI: 10.1039/C4Ib00050A  0.352
2014 Proctor A, Herrera-Loeza SG, Wang Q, Lawrence DS, Yeh JJ, Allbritton NL. Measurement of protein kinase B activity in single primary human pancreatic cancer cells. Analytical Chemistry. 86: 4573-80. PMID 24716819 DOI: 10.1021/Ac500616Q  0.569
2014 Ahmad AA, Wang Y, Gracz AD, Sims CE, Magness ST, Allbritton NL. Optimization of 3-D organotypic primary colonic cultures for organ-on-chip applications. Journal of Biological Engineering. 8: 9. PMID 24690469 DOI: 10.1186/1754-1611-8-9  0.409
2014 Wang Y, Ahmad AA, Sims CE, Magness ST, Allbritton NL. In vitro generation of colonic epithelium from primary cells guided by microstructures. Lab On a Chip. 14: 1622-31. PMID 24647645 DOI: 10.1039/C3Lc51353J  0.385
2014 Huang W, Proctor A, Sims CE, Allbritton NL, Zhang Q. Fluorous enzymatic synthesis of phosphatidylinositides. Chemical Communications (Cambridge, England). 50: 2928-31. PMID 24496473 DOI: 10.1039/C4Cc00022F  0.53
2014 Kovarik ML, Dickinson AJ, Roy P, Poonnen RA, Fine JP, Allbritton NL. Response of single leukemic cells to peptidase inhibitor therapy across time and dose using a microfluidic device. Integrative Biology : Quantitative Biosciences From Nano to Macro. 6: 164-74. PMID 24413844 DOI: 10.1039/C3Ib40249E  0.371
2014 Phillips RM, Dailey LA, Bair E, Samet JM, Allbritton NL. Ex vivo chemical cytometric analysis of protein tyrosine phosphatase activity in single human airway epithelial cells. Analytical Chemistry. 86: 1291-7. PMID 24380370 DOI: 10.1021/Ac403705C  0.418
2014 Gach PC, Attayek PJ, Whittlesey RL, Yeh JJ, Allbritton NL. Micropallet arrays for the capture, isolation and culture of circulating tumor cells from whole blood of mice engrafted with primary human pancreatic adenocarcinoma. Biosensors & Bioelectronics. 54: 476-83. PMID 24316450 DOI: 10.1016/J.Bios.2013.11.019  0.408
2014 Proctor A, Herrera-Loeza SG, Yeh JJ, Allbritton NL. Abstract 3345: Measurement of Akt activity in single primary human pancreatic cancer cells using chemical cytometry Cancer Research. 74: 3345-3345. DOI: 10.1158/1538-7445.Am2014-3345  0.627
2014 Alexis N, Wells H, Saini Y, Brighton L, Allbritton N, Muhlebach M. Application Of Isoraft Single Cell Isolation For Analysis Of Pediatric Bal Macrophages Journal of Allergy and Clinical Immunology. 133: AB142. DOI: 10.1016/J.Jaci.2013.12.523  0.347
2013 Shah PK, Hughes MR, Wang Y, Sims CE, Allbritton NL. Scalable synthesis of a biocompatible, transparent and superparamagnetic photoresist for microdevice fabrication. Journal of Micromechanics and Microengineering : Structures, Devices, and Systems. 23. PMID 24273390 DOI: 10.1088/0960-1317/23/10/107002  0.342
2013 Melvin AT, Woss GS, Park JH, Dumberger LD, Waters ML, Allbritton NL. A comparative analysis of the ubiquitination kinetics of multiple degrons to identify an ideal targeting sequence for a proteasome reporter. Plos One. 8: e78082. PMID 24205101 DOI: 10.1371/Journal.Pone.0078082  0.309
2013 Wang Y, Ahmad AA, Shah PK, Sims CE, Magness ST, Allbritton NL. Capture and 3D culture of colonic crypts and colonoids in a microarray platform. Lab On a Chip. 13: 4625-34. PMID 24113577 DOI: 10.1039/C3Lc50813G  0.413
2013 Wang Y, Phillips CN, Herrera GS, Sims CE, Yeh JJ, Allbritton NL. Array of Biodegradable Microraftsfor Isolation and Implantation of Living, Adherent Cells. Rsc Advances. 3: 9264-9272. PMID 23930219 DOI: 10.1039/C3Ra41764F  0.394
2013 Gach PC, Attayek PJ, Herrera G, Yeh JJ, Allbritton NL. Isolation and in vitro culture of rare cancer stem cells from patient-derived xenografts of pancreatic ductal adenocarcinoma. Analytical Chemistry. 85: 7271-8. PMID 23815678 DOI: 10.1021/Ac401165S  0.446
2013 Yang S, Proctor A, Cline LL, Houston KM, Waters ML, Allbritton NL. β-Turn sequences promote stability of peptide substrates for kinases within the cytosolic environment. The Analyst. 138: 4305-11. PMID 23785707 DOI: 10.1039/C3An00874F  0.652
2013 Melvin AT, Woss GS, Park JH, Waters ML, Allbritton NL. Measuring activity in the ubiquitin-proteasome system: from large scale discoveries to single cells analysis. Cell Biochemistry and Biophysics. 67: 75-89. PMID 23686610 DOI: 10.1007/S12013-013-9621-9  0.365
2013 Phillips RM, Bair E, Lawrence DS, Sims CE, Allbritton NL. Measurement of protein tyrosine phosphatase activity in single cells by capillary electrophoresis. Analytical Chemistry. 85: 6136-42. PMID 23682679 DOI: 10.1021/Ac401106E  0.363
2013 Kovarik ML, Shah PK, Armistead PM, Allbritton NL. Microfluidic chemical cytometry of peptide degradation in single drug-treated acute myeloid leukemia cells. Analytical Chemistry. 85: 4991-7. PMID 23590517 DOI: 10.1021/Ac4002029  0.452
2013 Dickinson AJ, Armistead PM, Allbritton NL. Automated capillary electrophoresis system for fast single-cell analysis. Analytical Chemistry. 85: 4797-804. PMID 23527995 DOI: 10.1021/Ac4005887  0.47
2013 Dobes NC, Dhopeshwarkar R, Henley WH, Ramsey JM, Sims CE, Allbritton NL. Laser-based directed release of array elements for efficient collection into targeted microwells. The Analyst. 138: 831-8. PMID 23223411 DOI: 10.1039/C2An36342A  0.786
2013 Kovarik ML, Ornoff DM, Melvin AT, Dobes NC, Wang Y, Dickinson AJ, Gach PC, Shah PK, Allbritton NL. Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field. Analytical Chemistry. 85: 451-72. PMID 23140554 DOI: 10.1021/Ac3031543  0.757
2013 Pai JH, Kluckman K, Cowley DO, Bortner DM, Sims CE, Allbritton NL. Efficient division and sampling of cell colonies using microcup arrays. The Analyst. 138: 220-8. PMID 23099535 DOI: 10.1039/C2An36065A  0.434
2013 Phillips RM, Lawrence DS, Sims CE, Allbritton NL. Abstract 4128: A novel approach to the measurement of tyrosine phosphorylation dynamics in intact single cells using capillary electrophoresis. Cancer Research. 73: 4128-4128. DOI: 10.1158/1538-7445.Am2013-4128  0.465
2012 Gach PC, Xu W, King SJ, Sims CE, Bear J, Allbritton NL. Microfabricated arrays for splitting and assay of clonal colonies. Analytical Chemistry. 84: 10614-20. PMID 23153031 DOI: 10.1021/Ac301895T  0.436
2012 Guo S, Wang Y, Allbritton N, Jiang X. Ultrasound-induced release of micropallets with cells. Applied Physics Letters. 101: 163703. PMID 23152640 DOI: 10.1063/1.4757648  0.368
2012 Wang K, Jiang D, Sims CE, Allbritton NL. Separation of fluorescently labeled phosphoinositides and sphingolipids by capillary electrophoresis. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 907: 79-86. PMID 23000742 DOI: 10.1016/J.Jchromb.2012.09.003  0.37
2012 Proctor A, Wang Q, Lawrence DS, Allbritton NL. Development of a peptidase-resistant substrate for single-cell measurement of protein kinase B activation. Analytical Chemistry. 84: 7195-202. PMID 22881604 DOI: 10.1021/Ac301489D  0.596
2012 Proctor A, Wang Q, Lawrence DS, Allbritton NL. Metabolism of peptide reporters in cell lysates and single cells. The Analyst. 137: 3028-38. PMID 22314840 DOI: 10.1039/C2An16162A  0.618
2012 Detwiler DA, Dobes NC, Sims CE, Kornegay JN, Allbritton NL. Polystyrene-coated micropallets for culture and separation of primary muscle cells. Analytical and Bioanalytical Chemistry. 402: 1083-91. PMID 22159513 DOI: 10.1007/S00216-011-5596-9  0.765
2012 Ma H, Mismar W, Wang Y, Small DW, Ras M, Allbritton NL, Sims CE, Venugopalan V. Impact of release dynamics of laser-irradiated polymer micropallets on the viability of selected adherent cells. Journal of the Royal Society, Interface / the Royal Society. 9: 1156-67. PMID 22158840 DOI: 10.1098/Rsif.2011.0691  0.609
2012 Wang Y, Shah P, Phillips C, Sims CE, Allbritton NL. Trapping cells on a stretchable microwell array for single-cell analysis. Analytical and Bioanalytical Chemistry. 402: 1065-72. PMID 22086401 DOI: 10.1007/S00216-011-5535-9  0.457
2012 Kovarik ML, Gach PC, Ornoff DM, Wang Y, Balowski J, Farrag L, Allbritton NL. Micro total analysis systems for cell biology and biochemical assays. Analytical Chemistry. 84: 516-40. PMID 21967743 DOI: 10.1021/Ac202611X  0.37
2012 Sims C, Wang Y, Gach P, Shah P, Allbritton N. Inexpensive Raft Arrays for Cell Patterning and Cloning Biophysical Journal. 102: 717a. DOI: 10.1016/J.Bpj.2011.11.3889  0.471
2012 Phillips RM, Lawrence DS, Allbritton NL. Analysis of EGFR Signaling in Single Cells using Capillary Electrophoresis Biophysical Journal. 102: 666a-667a. DOI: 10.1016/J.Bpj.2011.11.3629  0.438
2012 Proctor A, Wang Q, Lawrence DS, Allbritton NL. Construction of Peptidase-Resistant Substrates for Kinases Biophysical Journal. 102: 273a. DOI: 10.1016/J.Bpj.2011.11.1503  0.599
2012 Wang Y, Shah P, Phillips C, Sims CE, Allbritton NL. Erratum to: Trapping cells on a stretchable microwell array for single-cell analysis Analytical and Bioanalytical Chemistry. 404: 2519-2519. DOI: 10.1007/S00216-012-6266-2  0.37
2011 Gach PC, Wang Y, Phillips C, Sims CE, Allbritton NL. Isolation and manipulation of living adherent cells by micromolded magnetic rafts. Biomicrofluidics. 5: 32002-3200212. PMID 22007266 DOI: 10.1063/1.3608133  0.34
2011 Wang Y, Balowski J, Phillips C, Phillips R, Sims CE, Allbritton NL. Benchtop micromolding of polystyrene by soft lithography. Lab On a Chip. 11: 3089-97. PMID 21811715 DOI: 10.1039/C1Lc20281B  0.386
2011 Huang W, Jiang D, Wang X, Wang K, Sims CE, Allbritton NL, Zhang Q. Kinetic analysis of PI3K reactions with fluorescent PIP2 derivatives. Analytical and Bioanalytical Chemistry. 401: 1881-8. PMID 21789487 DOI: 10.1007/S00216-011-5257-Z  0.354
2011 Lai HH, Xu W, Allbritton NL. Use of a virtual wall valve in polydimethylsiloxane microfluidic devices for bioanalytical applications. Biomicrofluidics. 5: 24105. PMID 21629561 DOI: 10.1063/1.3584848  0.326
2011 Shadpour H, Zawistowski JS, Herman A, Hahn K, Allbritton NL. Patterning pallet arrays for cell selection based on high-resolution measurements of fluorescent biosensors. Analytica Chimica Acta. 696: 101-7. PMID 21621038 DOI: 10.1016/J.Aca.2011.04.012  0.423
2011 Phillips KS, Lai HH, Johnson E, Sims CE, Allbritton NL. Continuous analysis of dye-loaded, single cells on a microfluidic chip. Lab On a Chip. 11: 1333-41. PMID 21327264 DOI: 10.1039/C0Lc00370K  0.391
2011 Kovarik ML, Allbritton NL. Measuring enzyme activity in single cells. Trends in Biotechnology. 29: 222-30. PMID 21316781 DOI: 10.1016/J.Tibtech.2011.01.003  0.346
2011 Jiang D, Sims CE, Allbritton NL. Single-cell analysis of phosphoinositide 3-kinase and phosphatase and tensin homolog activation. Faraday Discussions. 149: 187-200; discussion . PMID 21221426 DOI: 10.1039/C005362G  0.424
2011 Xu W, Herman A, Phillips C, Pai JH, Sims CE, Allbritton NL. Selection and separation of viable cells based on a cell-lethal assay. Analytical Chemistry. 83: 278-83. PMID 21142138 DOI: 10.1021/Ac1023974  0.429
2011 Sims CE, Allbritton NL, Jiang D, Yang S, Proctor A, Phillips R. Measuring the Enzymatic Activity of Clinically Important Proteins in Single Cells Biophysical Journal. 100: 217a-218a. DOI: 10.1016/J.Bpj.2010.12.1401  0.515
2011 Allbritton N. Separating the One From the Many-Microfabricated Arrays for Cell Separations Biophysical Journal. 100: 189a. DOI: 10.1016/J.Bpj.2010.12.1249  0.484
2010 Wang Y, Phillips C, Xu W, Pai JH, Dhopeshwarkar R, Sims CE, Allbritton N. Micromolded arrays for separation of adherent cells. Lab On a Chip. 10: 2917-24. PMID 20838672 DOI: 10.1039/C0Lc00186D  0.485
2010 Pai JH, Xu W, Sims CE, Allbritton NL. Microtable arrays for culture and isolation of cell colonies. Analytical and Bioanalytical Chemistry. 398: 2595-604. PMID 20644916 DOI: 10.1007/S00216-010-3984-1  0.414
2010 Jiang D, Sims CE, Allbritton NL. Microelectrophoresis platform for fast serial analysis of single cells. Electrophoresis. 31: 2558-65. PMID 20603824 DOI: 10.1002/Elps.201000054  0.447
2010 Xu W, Luikart AM, Sims CE, Allbritton NL. Contact printing of arrayed microstructures. Analytical and Bioanalytical Chemistry. 397: 3377-85. PMID 20425106 DOI: 10.1007/S00216-010-3728-2  0.415
2010 Shadpour H, Allbritton NL. In situ roughening of polymeric microstructures. Acs Applied Materials & Interfaces. 2: 1086-93. PMID 20423129 DOI: 10.1021/Am900860S  0.336
2010 Wang Y, Dhopeshwarkar R, Najdi R, Waterman ML, Sims CE, Allbritton N. Microdevice to capture colon crypts for in vitro studies. Lab On a Chip. 10: 1596-603. PMID 20376386 DOI: 10.1039/B927316F  0.335
2010 Phillips KS, Kang KM, Licata L, Allbritton NL. Air-stable supported membranes for single-cell cytometry on PDMS microchips. Lab On a Chip. 10: 864-70. PMID 20300673 DOI: 10.1039/B921817C  0.313
2010 Xu W, Sims CE, Allbritton NL. Microcup arrays for the efficient isolation and cloning of cells. Analytical Chemistry. 82: 3161-7. PMID 20199088 DOI: 10.1021/Ac100434V  0.481
2010 Sims C, Allbritton N, Jiang D, Yang S, Proctor A, Phillips R. Single-Cell Biochemical Assays for the Molecular Targets of Disease Biophysical Journal. 98: 292a. DOI: 10.1016/J.Bpj.2009.12.1592  0.523
2010 Sims C, Allbritton N, Xu W, Wang Y, Shadpour H, Pai J, Dhopeshwarkar R, Gach P. Use of Arrays of Releasable Microstructures for Selection of Single Cells and Colonies Biophysical Journal. 98: 195a. DOI: 10.1016/J.Bpj.2009.12.1037  0.46
2010 Proctor A, Yang S, Kottegoda S, Brenner M, Phillips RM, Sims CE, Allbritton NL. Cell Signaling Studied at the Single-Cell Level Chemical Cytometry: Ultrasensitive Analysis of Single Cells. 31-54. DOI: 10.1002/9783527629640.ch3  0.646
2009 Fernandes N, Allbritton NL. Effect of the DEF motif on phosphorylation of peptide substrates by ERK. Biochemical and Biophysical Research Communications. 387: 414-8. PMID 19615338 DOI: 10.1016/J.Bbrc.2009.07.049  0.304
2009 Shadpour H, Sims CE, Allbritton NL. Enrichment and expansion of cells using antibody-coated micropallet arrays. Cytometry. Part a : the Journal of the International Society For Analytical Cytology. 75: 609-18. PMID 19504569 DOI: 10.1002/Cyto.A.20741  0.435
2009 Shadpour H, Sims CE, Thresher RJ, Allbritton NL. Sorting and expansion of murine embryonic stem cell colonies using micropallet arrays. Cytometry. Part a : the Journal of the International Society For Analytical Cytology. 75: 121-9. PMID 19012319 DOI: 10.1002/Cyto.A.20672  0.44
2008 Borland LM, Kottegoda S, Phillips KS, Allbritton NL. Chemical analysis of single cells. Annual Review of Analytical Chemistry (Palo Alto, Calif.). 1: 191-227. PMID 20636079 DOI: 10.1146/Annurev.Anchem.1.031207.113100  0.424
2008 Phillips KS, Kottegoda S, Kang KM, Sims CE, Allbritton NL. Separations in poly(dimethylsiloxane) microchips coated with supported bilayer membranes. Analytical Chemistry. 80: 9756-62. PMID 19006406 DOI: 10.1021/Ac801850Z  0.311
2008 Borland LM, Allbritton NL. Use of micellar electrokinetic chromatography to measure palmitoylation of a peptide. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 875: 451-8. PMID 18926781 DOI: 10.1016/J.Jchromb.2008.09.026  0.334
2008 Salazar GT, Wang Y, Sims CE, Bachman M, Li GP, Allbritton NL. Characterization of the laser-based release of micropallets from arrays. Journal of Biomedical Optics. 13: 034007. PMID 18601552 DOI: 10.1117/1.2937475  0.302
2008 Lai HH, Quinto-Su PA, Sims CE, Bachman M, Li GP, Venugopalan V, Allbritton NL. Characterization and use of laser-based lysis for cell analysis on-chip. Journal of the Royal Society, Interface / the Royal Society. 5: S113-21. PMID 18583277 DOI: 10.1098/Rsif.2008.0177.Focus  0.613
2008 Kottegoda S, Aoto PC, Sims CE, Allbritton NL. Biarsenical-tetracysteine motif as a fluorescent tag for detection in capillary electrophoresis. Analytical Chemistry. 80: 5358-66. PMID 18522433 DOI: 10.1021/Ac8003242  0.332
2008 Quinto-Su PA, To'a Salazar G, Sims CE, Allbritton NL, Venugopalan V. Mechanisms of pulsed laser microbeam release of SU-8 polymer "micropallets" for the collection and separation of adherent cells. Analytical Chemistry. 80: 4675-9. PMID 18489124 DOI: 10.1021/Ac800129A  0.574
2008 Wang Y, Salazar GT, Pai JH, Shadpour H, Sims CE, Allbritton NL. Micropallet arrays with poly(ethylene glycol) walls. Lab On a Chip. 8: 734-40. PMID 18432343 DOI: 10.1039/B800286J  0.387
2008 Marc PJ, Sims CE, Bachman M, Li GP, Allbritton NL. Fast-lysis cell traps for chemical cytometry. Lab On a Chip. 8: 710-6. PMID 18432340 DOI: 10.1039/B719301G  0.422
2008 Quinto-Su PA, Lai HH, Yoon HH, Sims CE, Allbritton NL, Venugopalan V. Examination of laser microbeam cell lysis in a PDMS microfluidic channel using time-resolved imaging. Lab On a Chip. 8: 408-14. PMID 18305858 DOI: 10.1039/B715708H  0.594
2008 Lee KJ, Mwongela SM, Kottegoda S, Borland L, Nelson AR, Sims CE, Allbritton NL. Determination of sphingosine kinase activity for cellular signaling studies. Analytical Chemistry. 80: 1620-7. PMID 18197698 DOI: 10.1021/Ac702305Q  0.398
2008 Allbritton N. Abstract ED08-02: Designing Life Chips and their impact for the prevention and early detection of cancer Cancer Prevention Research. 1. DOI: 10.1158/1940-6207.Prev-08-Ed08-02  0.47
2007 Nelson AR, Borland L, Allbritton NL, Sims CE. Myristoyl-based transport of peptides into living cells. Biochemistry. 46: 14771-81. PMID 18044965 DOI: 10.1021/Bi701295K  0.399
2007 Marc PJ, Sims CE, Allbritton NL. Coaxial flow system for chemical cytometry. Analytical Chemistry. 79: 9054-9. PMID 17979298 DOI: 10.1021/Ac7017519  0.434
2007 Pai JH, Wang Y, Salazar GT, Sims CE, Bachman M, Li GP, Allbritton NL. Photoresist with low fluorescence for bioanalytical applications. Analytical Chemistry. 79: 8774-80. PMID 17949059 DOI: 10.1021/Ac071528Q  0.334
2007 Fernandes N, Bailey DE, Vanvranken DL, Allbritton NL. Use of docking peptides to design modular substrates with high efficiency for mitogen-activated protein kinase extracellular signal-regulated kinase. Acs Chemical Biology. 2: 665-73. PMID 17918909 DOI: 10.1021/Cb700158Q  0.308
2007 Wang Y, Bachman M, Sims CE, Li GP, Allbritton NL. Stability of virtual air walls on micropallet arrays. Analytical Chemistry. 79: 7104-9. PMID 17705452 DOI: 10.1021/Ac070911S  0.33
2007 Nelson AR, Allbritton NL, Sims CE. Rapid sampling for single-cell analysis by capillary electrophoresis. Methods in Cell Biology. 82: 709-22. PMID 17586278 DOI: 10.1016/S0091-679X(06)82026-1  0.425
2007 Wang Y, Young G, Aoto PC, Pai JH, Bachman M, Li GP, Sims CE, Allbritton NL. Broadening cell selection criteria with micropallet arrays of adherent cells. Cytometry. Part a : the Journal of the International Society For Analytical Cytology. 71: 866-74. PMID 17559133 DOI: 10.1002/Cyto.A.20424  0.469
2007 Hellman AN, Rau KR, Yoon HH, Bae S, Palmer JF, Phillips KS, Allbritton NL, Venugopalan V. Laser-induced mixing in microfluidic channels. Analytical Chemistry. 79: 4484-92. PMID 17508715 DOI: 10.1021/Ac070081I  0.561
2007 Sims CE, Allbritton NL. Analysis of single mammalian cells on-chip. Lab On a Chip. 7: 423-40. PMID 17389958 DOI: 10.1039/B615235J  0.436
2007 Mwongela SM, Lee K, Sims CE, Allbritton NL. Separation of fluorescent phosphatidyl inositol phosphates by CE. Electrophoresis. 28: 1235-42. PMID 17366487 DOI: 10.1002/Elps.200600594  0.311
2007 Wang Y, Young G, Bachman M, Sims CE, Li GP, Allbritton NL. Collection and expansion of single cells and colonies released from a micropallet array. Analytical Chemistry. 79: 2359-66. PMID 17288466 DOI: 10.1021/Ac062180M  0.472
2007 Salazar GT, Wang Y, Young G, Bachman M, Sims CE, Li GP, Allbritton NL. Micropallet arrays for the separation of single, adherent cells. Analytical Chemistry. 79: 682-7. PMID 17222037 DOI: 10.1021/Ac0615706  0.465
2007 Sims CE, Bachman M, Li GP, Allbritton NL. Choosing one from the many: selection and sorting strategies for single adherent cells. Analytical and Bioanalytical Chemistry. 387: 5-8. PMID 16955263 DOI: 10.1007/S00216-006-0612-1  0.363
2006 Wang Y, Sims CE, Marc P, Bachman M, Li GP, Allbritton NL. Micropatterning of living cells on a heterogeneously wetted surface. Langmuir : the Acs Journal of Surfaces and Colloids. 22: 8257-62. PMID 16952271 DOI: 10.1021/La061602K  0.368
2006 Sims CE, Luzzi V, Allbritton NL. Localized sampling, electrophoresis, and biosensor analysis of Xenopus laevis cytoplasm for subcellular biochemical assays. Methods in Molecular Biology (Clifton, N.J.). 322: 413-24. PMID 16739740 DOI: 10.1007/978-1-59745-000-3_29  0.32
2006 Thomas M, Li GP, Bachman M, Sims C, Allbritton N, Venugopalan V. Effects of laser pulse energy, geometric confinement and material stiffness on laser-induced cell lysis in a microfluidic chip Biosilico. DOI: 10.1364/Bio.2006.Tui23  0.603
2005 Wang Y, Lai HH, Bachman M, Sims CE, Li GP, Allbritton NL. Covalent micropatterning of poly(dimethylsiloxane) by photografting through a mask. Analytical Chemistry. 77: 7539-46. PMID 16316160 DOI: 10.1021/Ac0509915  0.325
2004 Soughayer JS, Wang Y, Li H, Cheung SH, Rossi FM, Stanbridge EJ, Sims CE, Allbritton NL. Characterization of TAT-mediated transport of detachable kinase substrates. Biochemistry. 43: 8528-40. PMID 15222764 DOI: 10.1021/Bi036296D  0.384
2004 Li H, Sims CE, Kaluzova M, Stanbridge EJ, Allbritton NL. A quantitative single-cell assay for protein kinase B reveals important insights into the biochemical behavior of an intracellular substrate peptide. Biochemistry. 43: 1599-608. PMID 14769036 DOI: 10.1021/Bi035597K  0.419
2003 Hu S, Ren X, Bachman M, Sims CE, Li GP, Allbritton N. Cross-linked coatings for electrophoretic separations in poly(dimethylsiloxane) microchannels. Electrophoresis. 24: 3679-88. PMID 14613193 DOI: 10.1002/Elps.200305592  0.326
2003 McClain MA, Culbertson CT, Jacobson SC, Allbritton NL, Sims CE, Ramsey JM. Microfluidic devices for the high-throughput chemical analysis of cells. Analytical Chemistry. 75: 5646-55. PMID 14588001 DOI: 10.1021/Ac0346510  0.393
2003 Han F, Wang Y, Sims CE, Bachman M, Chang R, Li GP, Allbritton NL. Fast electrical lysis of cells for capillary electrophoresis. Analytical Chemistry. 75: 3688-96. PMID 14572031 DOI: 10.1021/Ac0341970  0.416
2003 Wang Y, Hu S, Li H, Allbritton NL, Sims CE. Separation of mixtures of acidic and basic peptides at neutral pH. Journal of Chromatography. A. 1004: 61-70. PMID 12929962 DOI: 10.1016/S0021-9673(03)00492-8  0.33
2003 Sims CE, Allbritton NL. Single-cell kinase assays: opening a window onto cell behavior. Current Opinion in Biotechnology. 14: 23-8. PMID 12565998 DOI: 10.1016/S0958-1669(02)00002-2  0.425
2001 Li H, Sims CE, Wu HY, Allbritton NL. Spatial control of cellular measurements with the laser micropipet. Analytical Chemistry. 73: 4625-31. PMID 11605840 DOI: 10.1021/Ac0105235  0.446
2001 Li H, Wu HY, Wang Y, Sims CE, Allbritton NL. Improved capillary electrophoresis conditions for the separation of kinase substrates by the laser micropipet system. Journal of Chromatography. B, Biomedical Sciences and Applications. 757: 79-88. PMID 11419751 DOI: 10.1016/S0378-4347(01)00080-9  0.376
2000 Soughayer JS, Krasieva T, Jacobson SC, Ramsey JM, Tromberg BJ, Allbritton NL. Characterization of cellular optoporation with distance. Analytical Chemistry. 72: 1342-7. PMID 10740880 DOI: 10.1021/Ac990982U  0.409
2000 Meredith GD, Sims CE, Soughayer JS, Allbritton NL. Measurement of kinase activation in single mammalian cells. Nature Biotechnology. 18: 309-12. PMID 10700147 DOI: 10.1038/73760  0.302
2000 Luzzi V, Murtazina D, Allbritton NL. Characterization of a biological detector cell for quantitation of inositol 1,4,5-trisphosphate Analytical Biochemistry. 277: 221-227. PMID 10625509 DOI: 10.1006/Abio.1999.4390  0.384
1998 Sims CE, Meredith GD, Krasieva TB, Berns MW, Tromberg BJ, Allbritton NL. Laser-micropipet combination for single-cell analysis. Analytical Chemistry. 70: 4570-7. PMID 9823716 DOI: 10.1021/Ac9802269  0.429
1998 Luzzi V, Sims CE, Soughayer JS, Allbritton NL. The physiologic concentration of inositol 1,4,5-trisphosphate in the oocytes of Xenopus laevis. The Journal of Biological Chemistry. 273: 28657-62. PMID 9786859 DOI: 10.1074/Jbc.273.44.28657  0.344
1997 Luzzi V, Lee CL, Allbritton NL. Localized Sampling of Cytoplasm from Xenopus Oocytes for Capillary Electrophoresis Analytical Chemistry. 69: 4761-4767. PMID 9406526 DOI: 10.1021/Ac970550O  0.348
1996 Fishman HA, Orwar O, Allbritton NL, Modi BP, Shear JB, Scheller RH, Zare RN. Cell-to-cell scanning in capillary electrophoresis. Analytical Chemistry. 68: 1181-6. PMID 8651490 DOI: 10.1021/Ac950904G  0.405
1995 Shear JB, Fishman HA, Allbritton NL, Garigan D, Zare RN, Scheller RH. Single cells as biosensors for chemical separations. Science (New York, N.Y.). 267: 74-7. PMID 7809609 DOI: 10.1126/Science.7809609  0.343
1995 Reinhard E, Yokoe H, Niebling KR, Allbritton NL, Kuhn MA, Meyer T. Localized calcium signals in early zebrafish development. Developmental Biology. 170: 50-61. PMID 7541377 DOI: 10.1006/Dbio.1995.1194  0.303
1993 Boniface JJ, Allbritton NL, Reay PA, Kantor RM, Stryer L, Davis MM. pH affects both the mechanism and the specificity of peptide binding to a class II major histocompatibility complex molecule. Biochemistry. 32: 11761-8. PMID 8218246 DOI: 10.1021/Bi00095A003  0.555
1992 Allbritton NL, Meyer T, Stryer L. Range of messenger action of calcium ion and inositol 1,4,5-trisphosphate. Science (New York, N.Y.). 258: 1812-5. PMID 1465619 DOI: 10.1126/Science.1465619  0.53
1988 Allbritton NL, Nagler-Anderson C, Elliott TJ, Verret CR, Eisen HN. Target cell lysis by cytotoxic T lymphocytes that lack detectable hemolytic perforin activity. Journal of Immunology (Baltimore, Md. : 1950). 141: 3243-8. PMID 3263417  0.54
1988 Nagler-Anderson C, Allbritton NL, Verret CR, Eisen HN. A comparison of the cytolytic properties of murine primary CD8+ cytotoxic T lymphocytes and cloned cytotoxic T cell lines. Immunological Reviews. 103: 111-25. PMID 3134291 DOI: 10.1111/J.1600-065X.1988.Tb00753.X  0.571
1988 Allbritton NL, Verret CR, Wolley RC, Eisen HN. Calcium ion concentrations and DNA fragmentation in target cell destruction by murine cloned cytotoxic T lymphocytes. The Journal of Experimental Medicine. 167: 514-27. PMID 2450162 DOI: 10.1084/Jem.167.2.514  0.553
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