1998 |
Zadina, James E |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Neurobiology of Endomorphis @ Tulane University of Louisiana
DESCRIPTION (Applicant's Abstract): The potent effects of morphine, a plant alkaloid, have long led to the assumption that the brain produces endogenous opioids. Several peptides with opioid properties and varying degrees of selectivity for the delta and kappa receptors have been discovers, whereas endogenous ligands with a clear selectivity for the mu receptor have remained elusive. We have recently isolated from brain two novel peptides, endomorphin-1 and -2, that have high affinity and selectivity for the mu opiate receptor (nature 386:499, 1997). We proposed to characterize the neurobiology of these newly discovered peptides. To determine whether they are produced endogenously from precursor proteins, we will clone and sequence the prohormone precursor(s) of these peptides and analyze the upstream region to identify key regulatory elements. Antibodies directed against each of the peptides have been generated and will be used to determine the distribution of the peptides within the nervous system, a crucial step toward understanding physiological functions of these peptides. Once the sequence of the gene(s) for the peptide is identified, riboprobes for in situ hybridization will be made and used to localize cells expressings the endomorphin genes. The possibility of a unique cell signaling profile will be tested with GTP gamma-S binding to measure capacity to activate G-proteins. Finally, the potential for abuse for the peptides will be tested in behavioral studies of opiate dependence and place preference. We take as our formal hypothesis that endomorphin-1 and -2 are endogenous, selective ligands for the mu receptor. Our specific aims are: 1. Isolate and sequence the gene(s) for endomorphin-1 and -2. 2. Demonstrate cellular localization of endomorphin protein and mRNA. 3. Determine the cellular signaling profile of the endomorphins. 4. Determine tolerance, dependence, and rewarding properties of endomorphins.
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1 |
1999 — 2000 |
Zadina, James E |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Neurobiology of Endomorphins @ Tulane University of Louisiana
DESCRIPTION (Applicant's Abstract): The potent effects of morphine, a plant alkaloid, have long led to the assumption that the brain produces endogenous opioids. Several peptides with opioid properties and varying degrees of selectivity for the delta and kappa receptors have been discovers, whereas endogenous ligands with a clear selectivity for the mu receptor have remained elusive. We have recently isolated from brain two novel peptides, endomorphin-1 and -2, that have high affinity and selectivity for the mu opiate receptor (nature 386:499, 1997). We proposed to characterize the neurobiology of these newly discovered peptides. To determine whether they are produced endogenously from precursor proteins, we will clone and sequence the prohormone precursor(s) of these peptides and analyze the upstream region to identify key regulatory elements. Antibodies directed against each of the peptides have been generated and will be used to determine the distribution of the peptides within the nervous system, a crucial step toward understanding physiological functions of these peptides. Once the sequence of the gene(s) for the peptide is identified, riboprobes for in situ hybridization will be made and used to localize cells expressings the endomorphin genes. The possibility of a unique cell signaling profile will be tested with GTP gamma-S binding to measure capacity to activate G-proteins. Finally, the potential for abuse for the peptides will be tested in behavioral studies of opiate dependence and place preference. We take as our formal hypothesis that endomorphin-1 and -2 are endogenous, selective ligands for the mu receptor. Our specific aims are: 1. Isolate and sequence the gene(s) for endomorphin-1 and -2. 2. Demonstrate cellular localization of endomorphin protein and mRNA. 3. Determine the cellular signaling profile of the endomorphins. 4. Determine tolerance, dependence, and rewarding properties of endomorphins.
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1 |
2021 |
Zadina, James E |
U18Activity Code Description: To provide support for testing, by means of a research design, the effectiveness of the transfer and application of techniques or interventions derived from a research base for the control of diseases or disorders or for the promotion of health. The project should be capable of making conclusions which are generalizable to other sites. These are usually cooperative programs between participating principal investigators, institutions, and the sponsoring Institute(s). |
Glycosylated Endomorphin Analogs For Treatment of Opioid Use Disorder (Oud) @ Tulane University of Louisiana
Opioids acting at the mu opioid receptor (MOR) remain the gold standard for moderate to severe pain relief, but serious side effects, particularly abuse liability, limit their use. The opioid epidemic has grown steadily in the past two decades, resulting in 130 deaths per day. Medications for treatment of opioid use disorder (OUD), particularly methadone and buprenorphine, have been helpful, but also have limitations. In addition to issues of compliance, they have reinforcing effects themselves, contributing to the need for close regulation. In this program, we will test modifications of a core pharmacophore of endomorphin analogs that has been shown to provide pain relief comparable to morphine while exhibiting reduction of several side effects, including abuse liability. In this program we will synthesize and screen glycosylated analogs of endomorphin with the goal of enhancing the blood-brain-barrier penetration and oral activity of the compounds. We will test them for antinociception and their ability to block the rewarding effects of morphine. The goal of these studies is to provide proof-of-concept that novel endomorphin analogs can provide 1.) Effective treatment of pain without rewarding properties, reducing the initiation of OUD and 2.) Effective blockade of opioid-induced reward, providing the basis for a novel medication for treatment of OUD.
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