| Year |
Citation |
Score |
| 2021 |
Li J, Lin J, Kohen A, Singh P, Francis K, Cheatum CM. Evolution of Optimized Hydride Transfer Reaction and Overall Enzyme Turnover in Human Dihydrofolate Reductase. Biochemistry. PMID 34875176 DOI: 10.1021/acs.biochem.1c00558 |
0.746 |
|
| 2021 |
Kholodar SA, Finer-Moore JS, Świderek K, Arafet K, Moliner V, Stroud RM, Kohen A. Caught in Action: X-ray Structure of Thymidylate Synthase with Noncovalent Intermediate Analog. Biochemistry. PMID 33829766 DOI: 10.1021/acs.biochem.1c00063 |
0.814 |
|
| 2020 |
Lansakara TI, Morris HS, Singh P, Kohen A, Tivanski AV. Rigid double-stranded DNA Linkers for Single Molecule Enzyme-Drug Interaction Measurements Using Molecular Recognition Force Spectroscopy. Langmuir : the Acs Journal of Surfaces and Colloids. PMID 32233509 DOI: 10.1021/Acs.Langmuir.9B03495 |
0.373 |
|
| 2019 |
Pagano P, Guo Q, Ranasinghe C, Schroeder E, Robben K, Häse F, Ye H, Wickersham K, Aspuru-Guzik A, Major DT, Gakhar L, Kohen A, Cheatum CM. Oscillatory Active-site Motions Correlate with Kinetic Isotope Effects in Formate Dehydrogenase. Acs Catalysis. 9: 11199-11206. PMID 33996196 DOI: 10.1021/acscatal.9b03345 |
0.833 |
|
| 2019 |
Ranasinghe C, Pagano PL, Sapienza PJ, Lee AL, Kohen A, Cheatum CM. Isotopic Labeling of Formate Dehydrogenase Perturbs the Protein Dynamics. The Journal of Physical Chemistry. B. PMID 31696711 DOI: 10.1021/Acs.Jpcb.9B08426 |
0.744 |
|
| 2019 |
Li J, Fortunato G, Lin J, Agarwal PK, Kohen A, Singh P, Cheatum CM. Evolution Conserves the Network of Coupled Residues in Dihydrofolate Reductase. Biochemistry. PMID 31423766 DOI: 10.1021/Acs.Biochem.9B00460 |
0.717 |
|
| 2019 |
Pagano P, Guo Q, Ranasinghe C, Schroeder E, Robben K, Häse F, Ye H, Wickersham K, Aspuru-Guzik A, Major DT, Gakhar L, Kohen A, Cheatum CM. Oscillatory Active-Site Motions Correlate with Kinetic Isotope Effects in Formate Dehydrogenase Acs Catalysis. 9: 11199-11206. DOI: 10.1021/Acscatal.9B03345 |
0.827 |
|
| 2018 |
Gurevic I, Islam Z, Świderek K, Trepka K, Ghosh AK, Moliner V, Kohen A. Experimental and Computational Studies Delineate the Role of Asparagine 177 in Hydride Transfer for E. coli Thymidylate Synthase. Acs Catalysis. 8: 10241-10253. PMID 31275729 DOI: 10.1021/Acscatal.8B02554 |
0.498 |
|
| 2018 |
Mhashal AR, Pshetitsky Y, Cheatum CM, Kohen A, Major DT. Evolutionary Effects on Bound Substrate pKa in Dihydrofolate Reductase. Journal of the American Chemical Society. PMID 30398861 DOI: 10.1021/Jacs.8B09089 |
0.734 |
|
| 2018 |
Kholodar SA, Ghosh AK, Świderek K, Moliner V, Kohen A. Parallel reaction pathways and noncovalent intermediates in thymidylate synthase revealed by experimental and computational tools. Proceedings of the National Academy of Sciences of the United States of America. PMID 30249644 DOI: 10.1073/Pnas.1811059115 |
0.811 |
|
| 2018 |
Mhashal AR, Pshetitsky Y, Eitan R, Cheatum CM, Kohen A, Major DT. Effect of Asp122 Mutation on the Hydride Transfer in E. Coli DHFR Demonstrates the Goldilocks of Enzyme Flexibility. The Journal of Physical Chemistry. B. PMID 30040418 DOI: 10.1021/Acs.Jpcb.8B05556 |
0.738 |
|
| 2018 |
Islam Z, Gurevic I, Strutzenberg TS, Ghosh AK, Iqbal T, Kohen A. Bacterial versus human thymidylate synthase: Kinetics and functionality. Plos One. 13: e0196506. PMID 29715278 DOI: 10.1371/Journal.Pone.0196506 |
0.493 |
|
| 2018 |
Mondal D, Koehn EM, Yao J, Wiemer DF, Kohen A. Chemo-enzymatic synthesis of the exocyclic olefin isomer of thymidine monophosphate. Bioorganic & Medicinal Chemistry. PMID 29606487 DOI: 10.1016/J.Bmc.2018.03.032 |
0.812 |
|
| 2017 |
Ranasinghe C, Guo Q, Sapienza PJ, Lee AL, Quinn DM, Cheatum CM, Kohen A. Protein Mass Effects on Formate Dehydrogenase. Journal of the American Chemical Society. PMID 29083897 DOI: 10.1021/Jacs.7B08359 |
0.785 |
|
| 2017 |
Kholodar SA, Ghosh AK, Kohen A. Measurement of Enzyme Isotope Effects. Methods in Enzymology. 596: 43-83. PMID 28911779 DOI: 10.1016/Bs.Mie.2017.06.033 |
0.84 |
|
| 2017 |
Karunaratne K, Luedtke N, Quinn DM, Kohen A. Flavin-dependent thymidylate synthase: N5 of flavin as a Methylene carrier. Archives of Biochemistry and Biophysics. PMID 28821425 DOI: 10.1016/J.Abb.2017.08.011 |
0.41 |
|
| 2017 |
Mhashal AR, Vardi-Kilshtain A, Kohen A, Major DT. The Role of the M20 Loop in the Hydride Transfer in E. Coli Dihydrofolate Reductase. The Journal of Biological Chemistry. PMID 28620051 DOI: 10.1074/Jbc.M117.777136 |
0.344 |
|
| 2017 |
Świderek K, Arafet K, Kohen A, Moliner V. Benchmarking Quantum Mechanics/Molecular Mechanics (QM/MM) Methods on the Thymidylate Synthase-Catalyzed Hydride Transfer. Journal of Chemical Theory and Computation. PMID 28192669 DOI: 10.1021/Acs.Jctc.6B01032 |
0.414 |
|
| 2017 |
Singh P, Guo Q, Kohen A. Chemoenzymatic Synthesis of Ubiquitous Biological Redox Cofactors Synlett. 28: 1151-1159. DOI: 10.1055/S-0036-1588768 |
0.493 |
|
| 2016 |
Abeysinghe T, Hong B, Wang Z, Kohen A. Preserved hydride transfer mechanism in evolutionarily divergent thymidylate synthases. Current Topics in Biochemical Research. 17: 19-30. PMID 28018055 |
0.674 |
|
| 2016 |
Singh P, Islam Z, Kohen A. Examinations of the Chemical Step in Enzyme Catalysis. Methods in Enzymology. 577: 287-318. PMID 27498642 DOI: 10.1016/Bs.Mie.2016.05.017 |
0.49 |
|
| 2016 |
Pshetitsky Y, Eitan R, Verner G, Kohen A, Major DT. Improved Sugar Puckering Profiles for Nicotinamide Ribonucleoside for Hybrid QM/MM Simulations. Journal of Chemical Theory and Computation. PMID 27490188 DOI: 10.1021/Acs.Jctc.6B00401 |
0.352 |
|
| 2016 |
Kholodar SA, Kohen A. Noncovalent Intermediate of Thymidylate Synthase: Fact or Fiction? Journal of the American Chemical Society. PMID 27327197 DOI: 10.1021/Jacs.6B03826 |
0.808 |
|
| 2016 |
Pagano P, Guo Q, Kohen A, Cheatum CM. Oscillatory Enzyme Dynamics Revealed by Two-Dimensional Infrared Spectroscopy. The Journal of Physical Chemistry Letters. PMID 27305279 DOI: 10.1021/Acs.Jpclett.6B01154 |
0.784 |
|
| 2016 |
Choi M, Karunaratne K, Kohen A. Flavin-Dependent Thymidylate Synthase as a New Antibiotic Target. Molecules (Basel, Switzerland). 21. PMID 27213314 DOI: 10.3390/Molecules21050654 |
0.444 |
|
| 2016 |
Guo Q, Gakhar L, Wickersham K, Francis K, Vardi-Kilshtain A, Major DT, Cheatum CM, Kohen A. Structural and Kinetic Studies of Formate Dehydrogenase from Candida boidinii. Biochemistry. PMID 27100912 DOI: 10.1021/Acs.Biochem.6B00181 |
0.825 |
|
| 2016 |
Mishanina TV, Yu L, Karunaratne K, Mondal D, Corcoran JM, Choi MA, Kohen A. An unprecedented mechanism of nucleotide methylation in organisms containing thyX. Science (New York, N.Y.). 351: 507-10. PMID 26823429 DOI: 10.1126/Science.Aad0300 |
0.464 |
|
| 2016 |
Francis K, Sapienza PJ, Lee AL, Kohen A. The Effect of the Protein Mass Modulation on Human Dihydrofolate Reductase. Biochemistry. PMID 26813442 DOI: 10.1021/Acs.Biochem.5B00945 |
0.518 |
|
| 2016 |
Kohen A. The enigmatic conservation of enzyme dynamics in evolution Perspectives in Science. 9: 60-66. DOI: 10.1016/J.Pisc.2016.03.023 |
0.454 |
|
| 2015 |
Singh P, Francis K, Kohen A. Network of remote and local protein dynamics in dihydrofolate reductase catalysis. Acs Catalysis. 5: 3067-3073. PMID 27182453 DOI: 10.1021/Acscatal.5B00331 |
0.458 |
|
| 2015 |
Kohen A. Dihydrofolate reductase as a model for studies of enzyme dynamics and catalysis. F1000research. 4. PMID 26918149 DOI: 10.12688/F1000Research.6968.1 |
0.479 |
|
| 2015 |
Islam Z, Strutzenberg TS, Ghosh AK, Kohen A. Activation of Two Sequential H-transfers in the Thymidylate Synthase Catalyzed Reaction. Acs Catalysis. 5: 6061-6068. PMID 26576323 DOI: 10.1021/Acscatal.5B01332 |
0.465 |
|
| 2015 |
Singh P, Morris H, Tivanski AV, Kohen A. A calibration curve for immobilized dihydrofolate reductase activity assay. Data in Brief. 4: 19-21. PMID 26217755 DOI: 10.1016/J.Dib.2015.04.005 |
0.366 |
|
| 2015 |
Mishanina TV, Kohen A. Synthesis and application of isotopically labeled flavin nucleotides. Journal of Labelled Compounds & Radiopharmaceuticals. 58: 370-5. PMID 26149960 DOI: 10.1002/Jlcr.3313 |
0.441 |
|
| 2015 |
Guo Q, Pagano P, Li YL, Kohen A, Cheatum CM. Line shape analysis of two-dimensional infrared spectra. The Journal of Chemical Physics. 142: 212427. PMID 26049447 DOI: 10.1063/1.4918350 |
0.695 |
|
| 2015 |
Ghosh AK, Islam Z, Krueger J, Abeysinghe T, Kohen A. The general base in the thymidylate synthase catalyzed proton abstraction. Physical Chemistry Chemical Physics : Pccp. PMID 25912171 DOI: 10.1039/C5Cp01246E |
0.459 |
|
| 2015 |
Swiderek K, Kohen A, Moliner V. The influence of active site conformations on the hydride transfer step of the thymidylate synthase reaction mechanism. Physical Chemistry Chemical Physics : Pccp. PMID 25868526 DOI: 10.1039/C5Cp01239B |
0.515 |
|
| 2015 |
Abeysinghe T, Kohen A. Role of long-range protein dynamics in different thymidylate synthase catalyzed reactions. International Journal of Molecular Sciences. 16: 7304-19. PMID 25837629 DOI: 10.3390/Ijms16047304 |
0.53 |
|
| 2015 |
Singh P, Morris H, Tivanski AV, Kohen A. Determination of concentration and activity of immobilized enzymes. Analytical Biochemistry. 484: 169-72. PMID 25707319 DOI: 10.1016/J.Ab.2015.02.014 |
0.428 |
|
| 2015 |
Singh P, Abeysinghe T, Kohen A. Linking protein motion to enzyme catalysis. Molecules (Basel, Switzerland). 20: 1192-209. PMID 25591120 DOI: 10.3390/Molecules20011192 |
0.497 |
|
| 2015 |
Kohen A. Role of dynamics in enzyme catalysis: substantial versus semantic controversies. Accounts of Chemical Research. 48: 466-73. PMID 25539442 DOI: 10.1021/Ar500322S |
0.392 |
|
| 2015 |
Doron D, Stojkovi? V, Gakhar L, Vardi-Kilshtain A, Kohen A, Major DT. Free energy simulations of active-site mutants of dihydrofolate reductase. The Journal of Physical Chemistry. B. 119: 906-16. PMID 25382260 DOI: 10.1021/Jp5059963 |
0.721 |
|
| 2015 |
Kohen A. Role of dynamics in enzyme catalysis: Substantial versus semantic controversies Accounts of Chemical Research. 48: 466-473. DOI: 10.1021/ar500322s |
0.32 |
|
| 2014 |
Liu CT, Francis K, Layfield JP, Huang X, Hammes-Schiffer S, Kohen A, Benkovic SJ. Escherichia coli dihydrofolate reductase catalyzed proton and hydride transfers: temporal order and the roles of Asp27 and Tyr100. Proceedings of the National Academy of Sciences of the United States of America. 111: 18231-6. PMID 25453098 DOI: 10.1073/Pnas.1415940111 |
0.511 |
|
| 2014 |
Klinman JP, Kohen A. Evolutionary aspects of enzyme dynamics. The Journal of Biological Chemistry. 289: 30205-12. PMID 25210031 DOI: 10.1074/Jbc.R114.565515 |
0.727 |
|
| 2014 |
Mishanina TV, Corcoran JM, Kohen A. Substrate activation in flavin-dependent thymidylate synthase. Journal of the American Chemical Society. 136: 10597-600. PMID 25025487 DOI: 10.1021/Ja506108B |
0.385 |
|
| 2014 |
Islam Z, Strutzenberg TS, Gurevic I, Kohen A. Concerted versus stepwise mechanism in thymidylate synthase. Journal of the American Chemical Society. 136: 9850-3. PMID 24949852 DOI: 10.1021/Ja504341G |
0.497 |
|
| 2014 |
Doron D, Kohen A, Nam K, Major DT. How Accurate Are Transition States from Simulations of Enzymatic Reactions? Journal of Chemical Theory and Computation. 10: 1863-1871. PMID 24860275 DOI: 10.1021/Ct5000742 |
0.441 |
|
| 2014 |
Wang Z, Singh P, Czekster CM, Kohen A, Schramm VL. Protein mass-modulated effects in the catalytic mechanism of dihydrofolate reductase: beyond promoting vibrations. Journal of the American Chemical Society. 136: 8333-41. PMID 24820793 DOI: 10.1021/Ja501936D |
0.646 |
|
| 2014 |
Roston D, Kohen A, Doron D, Major DT. Simulations of remote mutants of dihydrofolate reductase reveal the nature of a network of residues coupled to hydride transfer. Journal of Computational Chemistry. 35: 1411-7. PMID 24798860 DOI: 10.1002/Jcc.23629 |
0.788 |
|
| 2014 |
Francis K, Kohen A. Protein motions and the activation of the CH bond catalyzed by dihydrofolate reductase. Current Opinion in Chemical Biology. 21: 19-24. PMID 24742825 DOI: 10.1016/J.Cbpa.2014.03.009 |
0.499 |
|
| 2014 |
Singh P, Sen A, Francis K, Kohen A. Extension and limits of the network of coupled motions correlated to hydride transfer in dihydrofolate reductase Journal of the American Chemical Society. 136: 2575-2582. PMID 24450297 DOI: 10.1021/Ja411998H |
0.617 |
|
| 2014 |
Roston D, Kohen A. Stereospecific multiple isotopic labeling of benzyl alcohol Journal of Labelled Compounds and Radiopharmaceuticals. 57: 75-77. PMID 24327376 DOI: 10.1002/Jlcr.3143 |
0.788 |
|
| 2014 |
Roston D, Islam Z, Kohen A. Kinetic isotope effects as a probe of hydrogen transfers to and from common enzymatic cofactors Archives of Biochemistry and Biophysics. 544: 96-104. PMID 24161942 DOI: 10.1016/J.Abb.2013.10.010 |
0.806 |
|
| 2014 |
Klinman JP, Kohen A. Evolutionary aspects of enzyme dynamics Journal of Biological Chemistry. 289: 30205-30212. DOI: 10.1074/jbc.R114.565515 |
0.68 |
|
| 2014 |
Francis K, Kohen A. Standards for the reporting of kinetic isotope effects in enzymology Perspectives in Science. 1: 110-120. DOI: 10.1016/J.Pisc.2014.02.009 |
0.374 |
|
| 2013 |
Francis K, Stojković V, Kohen A. Preservation of protein dynamics in dihydrofolate reductase evolution Journal of Biological Chemistry. 288: 35961-35968. PMID 24158440 DOI: 10.1074/Jbc.M113.507632 |
0.785 |
|
| 2013 |
Roston D, Kohen A. A critical test of the "tunneling and coupled motion" concept in enzymatic alcohol oxidation Journal of the American Chemical Society. 135: 13624-13627. PMID 24020836 DOI: 10.1021/Ja405917M |
0.786 |
|
| 2013 |
Klinman JP, Kohen A. Hydrogen tunneling links protein dynamics to enzyme catalysis Annual Review of Biochemistry. 82: 471-496. PMID 23746260 DOI: 10.1146/Annurev-Biochem-051710-133623 |
0.727 |
|
| 2013 |
Roston D, Islam Z, Kohen A. Isotope effects as probes for enzyme catalyzed hydrogen-transfer reactions Molecules. 18: 5543-5567. PMID 23673528 DOI: 10.3390/Molecules18055543 |
0.821 |
|
| 2013 |
Wang Z, Sapienza PJ, Abeysinghe T, Luzum C, Lee AL, Finer-Moore JS, Stroud RM, Kohen A. Mg2+ binds to the surface of thymidylate synthase and affects hydride transfer at the interior active site. Journal of the American Chemical Society. 135: 7583-92. PMID 23611499 DOI: 10.1021/Ja400761X |
0.464 |
|
| 2013 |
Cheatum CM, Kohen A. Relationship of femtosecond-picosecond dynamics to enzyme-catalyzed H-transfer Topics in Current Chemistry. 337: 1-39. PMID 23539379 DOI: 10.1007/128_2012_407 |
0.762 |
|
| 2013 |
Wang Z, Ferrer S, Moliner V, Kohen A. QM/MM calculations suggest a novel intermediate following the proton abstraction catalyzed by thymidylate synthase. Biochemistry. 52: 2348-58. PMID 23464672 DOI: 10.1021/Bi400267Q |
0.511 |
|
| 2012 |
Vardi-Kilshtain A, Major DT, Kohen A, Engel H, Doron D. Hybrid Quantum and Classical Simulations of the Formate Dehydrogenase Catalyzed Hydride Transfer Reaction on an Accurate Semiempirical Potential Energy Surface. Journal of Chemical Theory and Computation. 8: 4786-4796. PMID 26605631 DOI: 10.1021/Ct300628E |
0.389 |
|
| 2012 |
Engel H, Doron D, Kohen A, Major DT. Momentum Distribution as a Fingerprint of Quantum Delocalization in Enzymatic Reactions: Open-Chain Path-Integral Simulations of Model Systems and the Hydride Transfer in Dihydrofolate Reductase. Journal of Chemical Theory and Computation. 8: 1223-34. PMID 26596739 DOI: 10.1021/Ct200874Q |
0.334 |
|
| 2012 |
Doron D, Kohen A, Major DT. Collective Reaction Coordinate for Hybrid Quantum and Molecular Mechanics Simulations: A Case Study of the Hydride Transfer in Dihydrofolate Reductase. Journal of Chemical Theory and Computation. 8: 2484-96. PMID 26588977 DOI: 10.1021/Ct300235K |
0.378 |
|
| 2012 |
Wang Z, Abeysinghe T, Finer-Moore JS, Stroud RM, Kohen A. A remote mutation affects the hydride transfer by disrupting concerted protein motions in thymidylate synthase. Journal of the American Chemical Society. 134: 17722-30. PMID 23034004 DOI: 10.1021/Ja307859M |
0.518 |
|
| 2012 |
Koehn EM, Perissinotti LL, Moghram S, Prabhakar A, Lesley SA, Mathews II, Kohen A. Folate binding site of flavin-dependent thymidylate synthase Proceedings of the National Academy of Sciences of the United States of America. 109: 15722-15727. PMID 23019356 DOI: 10.1073/Pnas.1206077109 |
0.808 |
|
| 2012 |
Sen A, Stojković V, Kohen A. Synthesis of radiolabeled nicotinamide cofactors from labeled pyridines: Versatile probes for enzyme kinetics Analytical Biochemistry. 430: 123-129. PMID 22922383 DOI: 10.1016/J.Ab.2012.08.012 |
0.795 |
|
| 2012 |
Roston D, Cheatum CM, Kohen A. Hydrogen Donor-Acceptor fluctuations from kinetic isotope effects: A phenomenological model Biochemistry. 51: 6860-6870. PMID 22857146 DOI: 10.1021/Bi300613E |
0.824 |
|
| 2012 |
Liu Q, Zhao Y, Hammann B, Eilers J, Lu Y, Kohen A. A model reaction assesses contribution of H-tunneling and coupled motions to enzyme catalysis. The Journal of Organic Chemistry. 77: 6825-33. PMID 22834675 DOI: 10.1021/Jo300879R |
0.483 |
|
| 2012 |
Wang Z, Roston D, Kohen A. Experimental and theoretical studies of enzyme-catalyzed hydrogen-transfer reactions. Advances in Protein Chemistry and Structural Biology. 87: 155-80. PMID 22607755 DOI: 10.1016/B978-0-12-398312-1.00006-8 |
0.821 |
|
| 2012 |
Mishanina TV, Koehn EM, Conrad JA, Palfey BA, Lesley SA, Kohen A. Trapping of an intermediate in the reaction catalyzed by flavin-dependent thymidylate synthase. Journal of the American Chemical Society. 134: 4442-8. PMID 22295882 DOI: 10.1021/Ja2120822 |
0.825 |
|
| 2012 |
Mishanina TV, Koehn EM, Kohen A. Mechanisms and inhibition of uracil methylating enzymes Bioorganic Chemistry. 43: 37-43. PMID 22172597 DOI: 10.1016/J.Bioorg.2011.11.005 |
0.808 |
|
| 2012 |
Stojkovi? V, Perissinotti LL, Willmer D, Benkovic SJ, Kohen A. Effects of the donor-acceptor distance and dynamics on hydride tunneling in the dihydrofolate reductase catalyzed reaction. Journal of the American Chemical Society. 134: 1738-45. PMID 22171795 DOI: 10.1021/Ja209425W |
0.524 |
|
| 2012 |
Dutta S, Li YL, Rock W, Houtman JC, Kohen A, Cheatum CM. 3-picolyl azide adenine dinucleotide as a probe of femtosecond to picosecond enzyme dynamics. The Journal of Physical Chemistry. B. 116: 542-8. PMID 22126535 DOI: 10.1021/Jp208677U |
0.759 |
|
| 2012 |
Kohen A. Enzyme Dynamics: Consensus and Controversy Journal of Biocatalysis & Biotransformation. 1. DOI: 10.4172/2324-9099.1000E103 |
0.327 |
|
| 2012 |
Dutta S, Li YL, Rock W, Houtman JCD, Kohen A, Cheatum CM. 3-picolyl azide adenine dinucleotide as a probe of femtosecond to picosecond enzyme dynamics Journal of Physical Chemistry B. 116: 542-548. DOI: 10.1021/jp208677u |
0.705 |
|
| 2011 |
Doron D, Major DT, Kohen A, Thiel W, Wu X. Hybrid Quantum and Classical Simulations of the Dihydrofolate Reductase Catalyzed Hydride Transfer Reaction on an Accurate Semi-Empirical Potential Energy Surface. Journal of Chemical Theory and Computation. 7: 3420-37. PMID 26598171 DOI: 10.1021/Ct2004808 |
0.439 |
|
| 2011 |
Oyeyemi OA, Sours KM, Lee T, Kohen A, Resing KA, Ahn NG, Klinman JP. Comparative hydrogen-deuterium exchange for a mesophilic vs thermophilic dihydrofolate reductase at 25 °c: Identification of a single active site region with enhanced flexibility in the mesophilic protein Biochemistry. 50: 8251-8260. PMID 21859100 DOI: 10.1021/Bi200640S |
0.789 |
|
| 2011 |
Dutta S, Rock W, Cook RJ, Kohen A, Cheatum CM. Two-dimensional infrared spectroscopy of azido-nicotinamide adenine dinucleotide in water. The Journal of Chemical Physics. 135: 055106. PMID 21823737 DOI: 10.1063/1.3623418 |
0.723 |
|
| 2011 |
Ditzler LR, Sen A, Gannon MJ, Kohen A, Tivanski AV. Self-assembled enzymatic monolayer directly bound to a gold surface: Activity and molecular recognition force spectroscopy studies Journal of the American Chemical Society. 133: 13284-13287. PMID 21809877 DOI: 10.1021/Ja205409V |
0.603 |
|
| 2011 |
Sen A, Yahashiri A, Kohen A. Triple isotopic labeling and kinetic isotope effects: Exposing H-transfer steps in enzymatic systems Biochemistry. 50: 6462-6468. PMID 21688781 DOI: 10.1021/Bi2003873 |
0.839 |
|
| 2011 |
Kanaan N, Ferrer S, Martà S, Garcia-Viloca M, Kohen A, Moliner V. Temperature dependence of the kinetic isotope effects in thymidylate synthase. A theoretical study. Journal of the American Chemical Society. 133: 6692-702. PMID 21476498 DOI: 10.1021/Ja1114369 |
0.473 |
|
| 2010 |
Stojkovi? V, Perissinotti LL, Lee J, Benkovic SJ, Kohen A. The effect of active-site isoleucine to alanine mutation on the DHFR catalyzed hydride-transfer. Chemical Communications (Cambridge, England). 46: 8974-6. PMID 20972508 DOI: 10.1039/C0Cc02988B |
0.369 |
|
| 2010 |
Bandaria JN, Dutta S, Nydegger MW, Rock W, Kohen A, Cheatum CM. Characterizing the dynamics of functionally relevant complexes of formate dehydrogenase Proceedings of the National Academy of Sciences of the United States of America. 107: 17974-17979. PMID 20876138 DOI: 10.1073/Pnas.0912190107 |
0.825 |
|
| 2010 |
Dutta S, Cook RJ, Houtman JCD, Kohen A, Cheatum CM. Characterization of azido-NAD+ to assess its potential as a two-dimensional infrared probe of enzyme dynamics Analytical Biochemistry. 407: 241-246. PMID 20705046 DOI: 10.1016/J.Ab.2010.08.008 |
0.746 |
|
| 2010 |
Wang Z, Kohen A. Thymidylate synthase catalyzed H-transfers: two chapters in one tale. Journal of the American Chemical Society. 132: 9820-5. PMID 20575541 DOI: 10.1021/Ja103010B |
0.479 |
|
| 2010 |
Roston D, Kohen A. Elusive transition state of alcohol dehydrogenase unveiled Proceedings of the National Academy of Sciences of the United States of America. 107: 9572-9577. PMID 20457944 DOI: 10.1073/Pnas.1000931107 |
0.77 |
|
| 2010 |
Koehn EM, Kohen A. Flavin-dependent thymidylate synthase: A novel pathway towards thymine Archives of Biochemistry and Biophysics. 493: 96-102. PMID 19643076 DOI: 10.1016/J.Abb.2009.07.016 |
0.814 |
|
| 2010 |
Sen A, Kohen A. Enzymatic tunneling and kinetic isotope effects: Chemistry at the crossroads Journal of Physical Organic Chemistry. 23: 613-619. DOI: 10.1002/Poc.1633 |
0.617 |
|
| 2009 |
Yahashiri A, Sen A, Kohen A. Microscale synthesis and kinetic isotope effect analysis of (4R)-[Ad-(14)C, 4-(2)H] NADPH and (4R)-[Ad-(3)H,4-(2)H] NADPH. Journal of Labelled Compounds & Radiopharmaceuticals. 52: 463-466. PMID 26045633 DOI: 10.1002/Jlcr.1660 |
0.788 |
|
| 2009 |
Yahashiri A, Nimrod G, Ben-Tal N, Howell EE, Kohen A. The effect of electrostatic shielding on H tunneling in R67 dihydrofolate reductase Chembiochem. 10: 2620-2623. PMID 19774544 DOI: 10.1002/Cbic.200900451 |
0.802 |
|
| 2009 |
Hill SE, Bandaria JN, Fox M, Vanderah E, Kohen A, Cheatum CM. Exploring the molecular origins of protein dynamics in the active site of human carbonic anhydrase II. The Journal of Physical Chemistry. B. 113: 11505-10. PMID 19637848 DOI: 10.1021/Jp901321M |
0.795 |
|
| 2009 |
Bandaria JN, Cheatum CM, Kohen A. Examination of enzymatic H-tunneling through kinetics and dynamics Journal of the American Chemical Society. 131: 10151-10155. PMID 19621965 DOI: 10.1021/Ja902120T |
0.838 |
|
| 2009 |
Wang Z, Chernyshev A, Koehn EM, Manuel TD, Lesley SA, Kohen A. Oxidase activity of a flavin-dependent thymidylate synthase. The Febs Journal. 276: 2801-10. PMID 19459936 DOI: 10.1111/J.1742-4658.2009.07003.X |
0.791 |
|
| 2009 |
Koehn EM, Fleischmann T, Conrad JA, Palfey BA, Lesley SA, Mathews II, Kohen A. An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX gene Nature. 458: 919-923. PMID 19370033 DOI: 10.1038/Nature07973 |
0.824 |
|
| 2009 |
Kanaan N, Martà S, Moliner V, Kohen A. QM/MM study of thymidylate synthase: enzymatic motions and the temperature dependence of the rate limiting step. The Journal of Physical Chemistry. A. 113: 2176-82. PMID 19182971 DOI: 10.1021/Jp810548D |
0.549 |
|
| 2009 |
Stojković V, Kohen A. Minireview: Enzymatic H transfers: Quantum tunneling and coupled motion from kinetic isotope effect studies Israel Journal of Chemistry. 49: 163-173. DOI: 10.1560/Ijc.49.2.163 |
0.765 |
|
| 2009 |
Hill SE, Bandaria JN, Fox M, Vanderah E, Kohen A, Cheatum CM. Exploring the molecular origins of protein dynamics in the active site of human carbonic anhydrase II Journal of Physical Chemistry B. 113: 11505-11510. DOI: 10.1021/jp901321m |
0.78 |
|
| 2009 |
Roston D, Kohen A. The Exit of the Tunnel: Yeast Alcohol Dehydrogenase from the Acceptor's Point of View Biophysical Journal. 96. DOI: 10.1016/J.Bpj.2008.12.2265 |
0.779 |
|
| 2009 |
Yahashiri A, Sen A, Kohen A. Microscale synthesis and kinetic isotope effect analysis of (4R)-[Ad- 14C,4-2H] NADPH and (4R)-[Ad-3H,4- 2H] NADPH Journal of Labelled Compounds and Radiopharmaceuticals. 52: 463-466. DOI: 10.1002/jlcr.1660 |
0.776 |
|
| 2008 |
Yahashiri A, Howell EE, Kohen A. Tuning of the H-transfer coordinate in primitive versus well-evolved enzymes Chemphyschem. 9: 980-982. PMID 18444258 DOI: 10.1002/Cphc.200800067 |
0.83 |
|
| 2008 |
Bandaria JN, Dutta S, Hill SE, Kohen A, Cheatum CM. Fast enzyme dynamics at the active site of formate dehydrogenase Journal of the American Chemical Society. 130: 22-23. PMID 18067303 DOI: 10.1021/Ja077599O |
0.832 |
|
| 2007 |
Hong B, Maley F, Kohen A. Role of Y94 in proton and hydride transfers catalyzed by thymidylate synthase Biochemistry. 46: 14188-14197. PMID 17999469 DOI: 10.1021/Bi701363S |
0.674 |
|
| 2007 |
Chernyshev A, Fleischmann T, Koehn EM, Lesley SA, Kohen A. The relationships between oxidase and synthase activities of flavin dependent thymidylate synthase (FDTS) Chemical Communications. 2861-2863. PMID 17609801 DOI: 10.1039/B700977A |
0.803 |
|
| 2007 |
Kanaan N, Martà S, Moliner V, Kohen A. A quantum mechanics/molecular mechanics study of the catalytic mechanism of the thymidylate synthase. Biochemistry. 46: 3704-13. PMID 17328531 DOI: 10.1021/Bi061953Y |
0.446 |
|
| 2007 |
Chernyshev A, Fleischmann T, Kohen A. Thymidyl biosynthesis enzymes as antibiotic targets Applied Microbiology and Biotechnology. 74: 282-289. PMID 17216455 DOI: 10.1007/S00253-006-0763-1 |
0.463 |
|
| 2006 |
Wang L, Goodey NM, Benkovic SJ, Kohen A. Coordinated effects of distal mutations on environmentally coupled tunneling in dihydrofolate reductase. Proceedings of the National Academy of Sciences of the United States of America. 103: 15753-8. PMID 17032759 DOI: 10.1073/Pnas.0606976103 |
0.518 |
|
| 2006 |
Limbach HH, Lopez JM, Kohen A. Arrhenius curves of hydrogen transfers: Tunnel effects, isotope effects and effects of pre-equilibria Philosophical Transactions of the Royal Society B: Biological Sciences. 361: 1399-1415. PMID 16873127 DOI: 10.1098/Rstb.2006.1872 |
0.436 |
|
| 2006 |
Wang L, Goodey NM, Benkovic SJ, Kohen A. The role of enzyme dynamics and tunnelling in catalysing hydride transfer: studies of distal mutants of dihydrofolate reductase. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. 361: 1307-15. PMID 16873118 DOI: 10.1098/Rstb.2006.1871 |
0.532 |
|
| 2006 |
Hong B, Haddad M, Maley F, Jensen JH, Kohen A. Hydride transfer versus hydrogen radical transfer in thymidylate synthase. Journal of the American Chemical Society. 128: 5636-7. PMID 16637621 DOI: 10.1021/Ja060196O |
0.643 |
|
| 2006 |
Mason A, Agrawal N, Washington MT, Lesley SA, Kohen A. A lag-phase in the reduction of flavin dependent thymidylate synthase (FDTS) revealed a mechanistic missing link. Chemical Communications (Cambridge, England). 1781-3. PMID 16609803 DOI: 10.1039/B517881A |
0.587 |
|
| 2006 |
Wang L, Tharp S, Selzer T, Benkovic SJ, Kohen A. Effects of a distal mutation on active site chemistry. Biochemistry. 45: 1383-92. PMID 16445280 DOI: 10.1021/Bi0518242 |
0.487 |
|
| 2006 |
Markham KA, Kohen A. Analytical procedures for the preparation, isolation, analysis and preservation of reduced nicotinamides Current Analytical Chemistry. 2: 379-388. DOI: 10.2174/157341106778520508 |
0.783 |
|
| 2005 |
Pu J, Ma S, Garcia-Viloca M, Gao J, Truhlar DG, Kohen A. Nonperfect synchronization of reaction center rehybridization in the transition state of the hydride transfer catalyzed by dihydrofolate reductase. Journal of the American Chemical Society. 127: 14879-86. PMID 16231943 DOI: 10.1021/Ja054170T |
0.388 |
|
| 2005 |
Hong B, Kohen A. Microscale synthesis of isotopically labeled 6R-N5, N 10 methylene-5, 6, 7, 8-tetrahydrofolate Journal of Labelled Compounds and Radiopharmaceuticals. 48: 759-769. DOI: 10.1002/Jlcr.993 |
0.64 |
|
| 2004 |
Sra AK, Hu Y, Martin GE, Snow DD, Ribbe MW, Kohen A. Competitive 15N kinetic isotope effects of nitrogenase-catalyzed dinitrogen reduction. Journal of the American Chemical Society. 126: 12768-9. PMID 15469259 DOI: 10.1021/Ja0458470 |
0.801 |
|
| 2004 |
Agrawal N, Lesley SA, Kuhn P, Kohen A. Mechanistic studies of a flavin-dependent thymidylate synthase. Biochemistry. 43: 10295-301. PMID 15301527 DOI: 10.1021/Bi0490439 |
0.711 |
|
| 2004 |
Agrawal N, Mihai C, Kohen A. Microscale synthesis of isotopically labeled R-[6-xH]N5,N10-methylene-5,6,7,8-tetrahydrofolate as a cofactor for thymidylate synthase. Analytical Biochemistry. 328: 44-50. PMID 15081906 DOI: 10.1016/J.Ab.2004.01.029 |
0.665 |
|
| 2004 |
Sikorski RS, Wang L, Markham KA, Rajagopalan PT, Benkovic SJ, Kohen A. Tunneling and coupled motion in the Escherichia coli dihydrofolate reductase catalysis. Journal of the American Chemical Society. 126: 4778-9. PMID 15080672 DOI: 10.1021/Ja031683W |
0.803 |
|
| 2004 |
Agrawal N, Hong B, Mihai C, Kohen A. Vibrationally enhanced hydrogen tunneling in the Escherichia coli thymidylate synthase catalyzed reaction. Biochemistry. 43: 1998-2006. PMID 14967040 DOI: 10.1021/Bi036124G |
0.725 |
|
| 2004 |
Markham KA, Sikorski RS, Kohen A. Synthesis and utility of 14C-labeled nicotinamide cofactors. Analytical Biochemistry. 325: 62-7. PMID 14715285 DOI: 10.1016/J.Ab.2003.10.027 |
0.804 |
|
| 2004 |
McCracken JA, Wang L, Kohen A. Synthesis of R and S tritiated reduced beta-nicotinamide adenine dinucleotide 2' phosphate. Analytical Biochemistry. 324: 131-6. PMID 14654055 DOI: 10.1016/J.Ab.2003.09.025 |
0.397 |
|
| 2003 |
Markham KA, Sikorski RS, Kohen A. Purification, analysis, and preservation of reduced nicotinamide adenine dinucleotide 2'-phosphate. Analytical Biochemistry. 322: 26-32. PMID 14705776 DOI: 10.1016/J.Ab.2003.07.010 |
0.818 |
|
| 2003 |
Agrawal N, Kohen A. Microscale synthesis of 2-tritiated isopropanol and 4R-tritiated reduced nicotinamide adenine dinucleotide phosphate. Analytical Biochemistry. 322: 179-84. PMID 14596825 DOI: 10.1016/J.Ab.2003.08.012 |
0.622 |
|
| 2003 |
Kohen A. Kinetic isotope effects as probes for hydrogen tunneling, coupled motion and dynamics contributions to enzyme catalysis Progress in Reaction Kinetics and Mechanism. 28: 119-156. DOI: 10.3184/007967403103165486 |
0.467 |
|
| 2002 |
Kohen A, Jensen JH. Boundary conditions for the Swain-Schaad relationship as a criterion for hydrogen tunneling. Journal of the American Chemical Society. 124: 3858-64. PMID 11942822 DOI: 10.1021/Ja016909E |
0.405 |
|
| 2001 |
Truhlar D, Kohen A. Convex Arrhenius plots and their interpretation. Proceedings of the National Academy of Sciences of the United States of America. 98: 848-51. PMID 11158559 DOI: 10.1073/Pnas.98.3.848 |
0.411 |
|
| 2000 |
Kohen A, Klinman JP. Protein flexibility correlates with degree of hydrogen tunneling in thermophilic and mesophilic alcohol dehydrogenases [18] Journal of the American Chemical Society. 122: 10738-10739. DOI: 10.1021/Ja002229K |
0.6 |
|
| 1999 |
Kohen A, Klinman JP. Hydrogen tunneling in biology. Chemistry & Biology. 6: R191-8. PMID 10381408 DOI: 10.1016/S1074-5521(99)80058-1 |
0.649 |
|
| 1999 |
Kohen A, Cannio R, Bartolucci S, Klinman JP. Enzyme dynamics and hydrogen tunnelling in a thermophilic alcohol dehydrogenase. Nature. 399: 496-9. PMID 10365965 DOI: 10.1038/20981 |
0.735 |
|
| 1998 |
Liang PH, Lewis J, Anderson KS, Kohen A, D'Souza FW, Benenson Y, Baasov T. Catalytic mechanism of Kdo8P synthase: transient kinetic studies and evaluation of a putative reaction intermediate. Biochemistry. 37: 16390-9. PMID 9819231 DOI: 10.1021/Bi981374W |
0.537 |
|
| 1998 |
Kohen A, Klinman JP. Enzyme Catalysis: Beyond Classical Paradigms Accounts of Chemical Research. 31: 397-404. DOI: 10.1021/Ar9701225 |
0.753 |
|
| 1997 |
Kohen A, Jonsson T, Klinman JP. Effects of protein glycosylation on catalysis: changes in hydrogen tunneling and enthalpy of activation in the glucose oxidase reaction. Biochemistry. 36: 2603-11. PMID 9054567 DOI: 10.1021/Bi962492R |
0.725 |
|
| 1997 |
Kohen A, Jonsson T, Klinman JP. Effects of enzyme glycosylation on the chemical step of catalysis, as probed by hydrogen tunneling and enthalpy of activation Techniques in Protein Chemistry. 8: 311-319. DOI: 10.1016/S1080-8914(97)80032-4 |
0.742 |
|
| 1997 |
Liang PH, Kohen A, Baasov T, Anderson KS. Catalytic mechanism of Kdo8P synthase. Pre-steady-state kinetic analysis using rapid chemical quench flow methods Bioorganic and Medicinal Chemistry Letters. 7: 2463-2468. DOI: 10.1016/S0960-894X(97)10021-X |
0.37 |
|
| 1995 |
Baasov T, Kohen A. Synthesis, inhibition, and acid-catalyzed hydrolysis studies of model compounds of the proposed intermediate in the Kdo8P-synthase-catalyzed reaction Journal of the American Chemical Society. 117: 6165-6172. DOI: 10.1021/Ja00128A002 |
0.36 |
|
| 1994 |
Kohen A, Belakhov V, Baasov T. Towards the synthesis of the putative reaction intermediate in the Kdo8P synthase-catalyzed reaction. Synthesis and evaluation of 3-deoxy-d-manno-2-octulosonate-2-phosphate Tetrahedron Letters. 35: 3179-3182. DOI: 10.1016/S0040-4039(00)76861-4 |
0.366 |
|
| 1993 |
Baasov T, Sheffer-Dee-Noor S, Kohen A, Jakob A, Belakhov V. Catalytic mechanism of 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase. The use of synthetic analogues to probe the structure of the putative reaction intermediate. European Journal of Biochemistry / Febs. 217: 991-9. PMID 8223657 DOI: 10.1111/J.1432-1033.1993.Tb18330.X |
0.441 |
|
| 1993 |
Kohen A, Berkovich R, Belakhov V, Baasov T. Stereochemistry of the KDO8P synthase. An efficient synthesis of the 3-fluoro analogues of KDO8P Bioorganic and Medicinal Chemistry Letters. 3: 1577-1582. DOI: 10.1016/S0960-894X(00)80021-9 |
0.391 |
|
| 1992 |
Kohen A, Jakob A, Baasov T. Mechanistic studies of 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase from Escherichia coli. European Journal of Biochemistry / Febs. 208: 443-9. PMID 1521535 DOI: 10.1111/J.1432-1033.1992.Tb17206.X |
0.434 |
|
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