Takeharu Nagai - Publications

Affiliations: 
Osaka University, Toyonaka-shi, Ōsaka-fu, Japan 

63 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2022 Jahan A, Akter MT, Takemoto K, Oura T, Shitara A, Semba S, Nezu A, Suto S, Nagai T, Tanimura A. Insertion of circularly permuted cyan fluorescent protein into the ligand-binding domain of inositol 1,4,5-trisphosphate receptor for enhanced FRET upon binding of fluorescent ligand. Cell Calcium. 108: 102668. PMID 36335765 DOI: 10.1016/j.ceca.2022.102668  0.311
2022 Sugiura K, Nagai T. Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application. Communications Biology. 5: 1172. PMID 36329112 DOI: 10.1038/s42003-022-04153-7  0.348
2021 Wazawa T, Noma R, Uto S, Sugiura K, Washio T, Nagai T. A Photoswitchable Fluorescent Protein for Hours-Time-Lapse and Sub-Second-Resolved Super-Resolution Imaging. Microscopy (Oxford, England). PMID 33481018 DOI: 10.1093/jmicro/dfab001  0.321
2019 Lu K, Vu CQ, Matsuda T, Nagai T. Fluorescent Protein-Based Indicators for Functional Super-Resolution Imaging of Biomolecular Activities in Living Cells. International Journal of Molecular Sciences. 20. PMID 31744242 DOI: 10.3390/ijms20225784  0.363
2019 Farhana I, Hossain MN, Suzuki K, Matsuda T, Nagai T. Genetically Encoded Fluorescence/Bioluminescence Bimodal Indicators for Ca Imaging. Acs Sensors. PMID 31276380 DOI: 10.1021/acssensors.9b00531  0.415
2018 Arai Y, Takauchi H, Ogami Y, Fujiwara S, Nakano M, Matsuda T, Nagai T. Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging. Acs Chemical Biology. 13: 1938-1943. PMID 29963852 DOI: 10.1021/acschembio.8b00200  0.308
2018 Riani YD, Matsuda T, Takemoto K, Nagai T. Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation. Bmc Biology. 16: 50. PMID 29712573 DOI: 10.1186/s12915-018-0514-7  0.318
2018 Hara-Kuge S, Nishihara T, Matsuda T, Kitazono T, Teramoto T, Nagai T, Ishihara T. An improved inverse-type Ca2+ indicator can detect putative neuronal inhibition in Caenorhabditis elegans by increasing signal intensity upon Ca2+ decrease. Plos One. 13: e0194707. PMID 29694380 DOI: 10.1371/journal.pone.0194707  0.322
2018 Hossain MN, Suzuki K, Iwano M, Matsuda T, Nagai T. Bioluminescent low-affinity Caindicator for ER with multicolor calcium imaging in single living cells. Acs Chemical Biology. PMID 29494125 DOI: 10.1021/acschembio.7b01014  0.36
2017 Suzuki M, Sato M, Koyama H, Hara Y, Hayashi K, Yasue N, Imamura H, Fujimori T, Nagai T, Campbell RE, Ueno N. Distinct intracellular Ca(2+) dynamics regulate apical constriction and differentially contribute to neural tube closure. Development (Cambridge, England). PMID 28219946 DOI: 10.1242/Dev.141952  0.307
2017 Kim SY, Arai Y, Tani T, Takatsuka H, Saito Y, Kawashima T, Kawakami S, Miyawaki A, Nagai T. Simultaneous imaging of multiple cellular events using high-accuracy fluorescence polarization microscopy. Microscopy (Oxford, England). PMID 28043995 DOI: 10.1093/jmicro/dfw110  0.586
2016 Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, Nagai T. Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nature Communications. 7: 13718. PMID 27966527 DOI: 10.1038/ncomms13718  0.361
2016 Saito K, Nakano M, Nagai T. Luminescence Imaging: (a) Multicolor Visualization of Ca(2+) Dynamics in Different Cellular Compartments and (b) Video-Rate Tumor Detection in a Freely Moving Mouse. Methods in Molecular Biology (Clifton, N.J.). 1461: 289-97. PMID 27424914 DOI: 10.1007/978-1-4939-3813-1_24  0.304
2016 Lissek T, Obenhaus HA, Ditzel DA, Nagai T, Miyawaki A, Sprengel R, Hasan MT. General Anesthetic Conditions Induce Network Synchrony and Disrupt Sensory Processing in the Cortex. Frontiers in Cellular Neuroscience. 10: 64. PMID 27147963 DOI: 10.3389/Fncel.2016.00064  0.443
2016 Morikawa TJ, Fujita H, Kitamura A, Horio T, Yamamoto J, Kinjo M, Sasaki A, Machiyama H, Yoshizawa K, Ichimura T, Imada K, Nagai T, Watanabe TM. Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it. Scientific Reports. 6: 22342. PMID 26956628 DOI: 10.1038/srep22342  0.374
2015 Iwano M, Ito K, Fujii S, Kakita M, Asano-Shimosato H, Igarashi M, Kaothien-Nakayama P, Entani T, Kanatani A, Takehisa M, Tanaka M, Komatsu K, Shiba H, Nagai T, Miyawaki A, et al. Calcium signalling mediates self-incompatibility response in the Brassicaceae. Nature Plants. 1: 15128. PMID 27250681 DOI: 10.1038/nplants.2015.128  0.483
2015 Uno SN, Tiwari DK, Kamiya M, Arai Y, Nagai T, Urano Y. A guide to use photocontrollable fluorescent proteins and synthetic smart fluorophores for nanoscopy. Microscopy (Oxford, England). 64: 263-77. PMID 26152215 DOI: 10.1093/jmicro/dfv037  0.329
2015 Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, Fujita K, Nagai T. A fast- and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy. Nature Methods. 12: 515-8. PMID 25894946 DOI: 10.1038/nmeth.3362  0.313
2015 Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin T, Okada Y, Nagai T. Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proceedings of the National Academy of Sciences of the United States of America. 112: 4352-6. PMID 25831507 DOI: 10.1073/pnas.1418468112  0.358
2014 Oshima Y, Imamura T, Shintani A, Kajiura-Kobayashi H, Hibi T, Nagai T, Nonaka S, Nemoto T. Ultrasensitive imaging of Ca2+ dynamics in pancreatic acinar cells of yellow cameleon-nano transgenic mice. International Journal of Molecular Sciences. 15: 19971-86. PMID 25372943 DOI: 10.3390/ijms151119971  0.374
2014 Matsuda T, Nagai T. Quantitative measurement of intracellular protein dynamics using photobleaching or photoactivation of fluorescent proteins. Microscopy (Oxford, England). 63: 403-8. PMID 25268018 DOI: 10.1093/jmicro/dfu033  0.316
2013 Yamanaka M, Saito K, Smith NI, Kawata S, Nagai T, Fujita K. Saturated excitation of fluorescent proteins for subdiffraction-limited imaging of living cells in three dimensions. Interface Focus. 3: 20130007. PMID 24511385 DOI: 10.1098/rsfs.2013.0007  0.35
2013 Miyawaki A, Nagai T, Mizuno H. Imaging intracellular free Ca2+ concentration using yellow cameleons. Cold Spring Harbor Protocols. 2013. PMID 24184769 DOI: 10.1101/pdb.prot078642  0.664
2013 Tiwari DK, Nagai T. Smart fluorescent proteins: innovation for barrier-free superresolution imaging in living cells. Development, Growth & Differentiation. 55: 491-507. PMID 23635320 DOI: 10.1111/dgd.12064  0.305
2013 Horikawa K, Nagai T. [Spatiotemporal regulation of Ca(2 +) dynamics in live cells revealed by Ca(2 +) imaging]. Clinical Calcium. 23: 527-33. PMID 23545742  0.309
2013 Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li WH, et al. Improved orange and red Ca²± indicators and photophysical considerations for optogenetic applications. Acs Chemical Neuroscience. 4: 963-72. PMID 23452507 DOI: 10.1021/Cn400012B  0.402
2013 Hoi H, Matsuda T, Nagai T, Campbell RE. Highlightable Ca2+ indicators for live cell imaging. Journal of the American Chemical Society. 135: 46-9. PMID 23256581 DOI: 10.1021/Ja310184A  0.424
2012 Kominami K, Nagai T, Sawasaki T, Tsujimura Y, Yashima K, Sunaga Y, Tsuchimochi M, Nishimura J, Chiba K, Nakabayashi J, Koyamada K, Endo Y, Yokota H, Miyawaki A, Manabe N, et al. In vivo imaging of hierarchical spatiotemporal activation of caspase-8 during apoptosis. Plos One. 7: e50218. PMID 23185580 DOI: 10.1371/journal.pone.0050218  0.419
2012 Iwano M, Ngo QA, Entani T, Shiba H, Nagai T, Miyawaki A, Isogai A, Grossniklaus U, Takayama S. Cytoplasmic Ca2+ changes dynamically during the interaction of the pollen tube with synergid cells. Development (Cambridge, England). 139: 4202-9. PMID 23093426 DOI: 10.1242/Dev.081208  0.522
2012 Kominami K, Nakabayashi J, Nagai T, Tsujimura Y, Chiba K, Kimura H, Miyawaki A, Sawasaki T, Yokota H, Manabe N, Sakamaki K. The molecular mechanism of apoptosis upon caspase-8 activation: quantitative experimental validation of a mathematical model. Biochimica Et Biophysica Acta. 1823: 1825-40. PMID 22801217 DOI: 10.1016/j.bbamcr.2012.07.003  0.452
2012 Chang YF, Arai Y, Nagai T. Optogenetic activation during detector "dead time" enables compatible real-time fluorescence imaging. Neuroscience Research. 73: 341-7. PMID 22652483 DOI: 10.1016/j.neures.2012.05.007  0.31
2011 Yamada Y, Michikawa T, Hashimoto M, Horikawa K, Nagai T, Miyawaki A, Häusser M, Mikoshiba K. Quantitative comparison of genetically encoded Ca indicators in cortical pyramidal cells and cerebellar Purkinje cells. Frontiers in Cellular Neuroscience. 5: 18. PMID 21994490 DOI: 10.3389/fncel.2011.00018  0.608
2011 Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE. An expanded palette of genetically encoded Ca²⁺ indicators. Science (New York, N.Y.). 333: 1888-91. PMID 21903779 DOI: 10.1126/Science.1208592  0.41
2011 Nakano M, Imamura H, Nagai T, Noji H. Ca2+ Regulation of Mitochondrial ATP Synthesis Visualized at the Single Cell Level Acs Chemical Biology. 6: 709-715. PMID 21488691 DOI: 10.1021/Cb100313N  0.409
2011 Takemoto K, Matsuda T, McDougall M, Klaubert DH, Hasegawa A, Los GV, Wood KV, Miyawaki A, Nagai T. Chromophore-assisted light inactivation of HaloTag fusion proteins labeled with eosin in living cells Acs Chemical Biology. 6: 401-406. PMID 21226520 DOI: 10.1021/cb100431e  0.476
2010 Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura T, Miyawaki A, Michikawa T, Mikoshiba K, Nagai T. Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano. Nature Methods. 7: 729-32. PMID 20693999 DOI: 10.1038/nmeth.1488  0.589
2010 Lütcke H, Murayama M, Hahn T, Margolis DJ, Astori S, Zum Alten Borgloh SM, Göbel W, Yang Y, Tang W, Kügler S, Sprengel R, Nagai T, Miyawaki A, Larkum ME, Helmchen F, et al. Optical recording of neuronal activity with a genetically-encoded calcium indicator in anesthetized and freely moving mice. Frontiers in Neural Circuits. 4: 9. PMID 20461230 DOI: 10.3389/Fncir.2010.00009  0.529
2010 Saito K, Hatsugai N, Horikawa K, Kobayashi K, Matsu-Ura T, Mikoshiba K, Nagai T. Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level. Plos One. 5: e9935. PMID 20376337 DOI: 10.1371/journal.pone.0009935  0.521
2010 Hong JH, Min CH, Jeong B, Kojiya T, Morioka E, Nagai T, Ikeda M, Lee KJ. Intracellular calcium spikes in rat suprachiasmatic nucleus neurons induced by BAPTA-based calcium dyes. Plos One. 5: e9634. PMID 20224788 DOI: 10.1371/Journal.Pone.0009634  0.314
2009 Iwano M, Entani T, Shiba H, Kakita M, Nagai T, Mizuno H, Miyawaki A, Shoji T, Kubo K, Isogai A, Takayama S. Fine-tuning of the cytoplasmic Ca2+ concentration is essential for pollen tube growth. Plant Physiology. 150: 1322-34. PMID 19474213 DOI: 10.1104/pp.109.139329  0.647
2009 Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa K, Nagai T. An ultramarine fluorescent protein with increased photostability and pH insensitivity. Nature Methods. 6: 351-3. PMID 19349978 DOI: 10.1038/nmeth.1317  0.379
2009 Matsuda T, Nagai T. [Bioimaging and analysis of protein dynamics with a novel photoswichable fluorescent protein probe]. Tanpakushitsu Kakusan Koso. Protein, Nucleic Acid, Enzyme. 53: 1858-64. PMID 19044022  0.313
2008 Sunabori T, Tokunaga A, Nagai T, Sawamoto K, Okabe M, Miyawaki A, Matsuzaki Y, Miyata T, Okano H. Cell-cycle-specific nestin expression coordinates with morphological changes in embryonic cortical neural progenitors. Journal of Cell Science. 121: 1204-12. PMID 18349072 DOI: 10.1242/Jcs.025064  0.456
2008 Matsuda T, Miyawaki A, Nagai T. Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein. Nature Methods. 5: 339-45. PMID 18345008 DOI: 10.1038/nmeth.1193  0.535
2007 Takemoto K, Kuranaga E, Tonoki A, Nagai T, Miyawaki A, Miura M. Local initiation of caspase activation in Drosophila salivary gland programmed cell death in vivo. Proceedings of the National Academy of Sciences of the United States of America. 104: 13367-72. PMID 17679695 DOI: 10.1073/pnas.0702733104  0.435
2006 Nagai T. [Various applications of fluorescent proteins for cell biology]. Seikagaku. the Journal of Japanese Biochemical Society. 78: 759-63. PMID 16986726  0.348
2006 Kazuno AA, Munakata K, Nagai T, Shimozono S, Tanaka M, Yoneda M, Kato N, Miyawaki A, Kato T. Identification of mitochondrial DNA polymorphisms that alter mitochondrial matrix pH and intracellular calcium dynamics. Plos Genetics. 2: e128. PMID 16895436 DOI: 10.1371/journal.pgen.0020128  0.418
2005 Kohyama J, Tokunaga A, Fujita Y, Miyoshi H, Nagai T, Miyawaki A, Nakao K, Matsuzaki Y, Okano H. Visualization of spatiotemporal activation of Notch signaling: live monitoring and significance in neural development. Developmental Biology. 286: 311-25. PMID 16153632 DOI: 10.1016/J.Ydbio.2005.08.003  0.458
2005 Miyawaki A, Nagai T, Mizuno H. Engineering fluorescent proteins. Advances in Biochemical Engineering/Biotechnology. 95: 1-15. PMID 16080263 DOI: 10.1007/b102208  0.693
2005 Takao K, Okamoto K, Nakagawa T, Neve RL, Nagai T, Miyawaki A, Hashikawa T, Kobayashi S, Hayashi Y. Visualization of synaptic Ca2+ /calmodulin-dependent protein kinase II activity in living neurons. The Journal of Neuroscience : the Official Journal of the Society For Neuroscience. 25: 3107-12. PMID 15788767 DOI: 10.1523/Jneurosci.0085-05.2005  0.526
2005 Lin X, Várnai P, Csordás G, Balla A, Nagai T, Miyawaki A, Balla T, Hajnóczky G. Control of calcium signal propagation to the mitochondria by inositol 1,4,5-trisphosphate-binding proteins. The Journal of Biological Chemistry. 280: 12820-32. PMID 15644334 DOI: 10.1074/Jbc.M411591200  0.518
2004 Iwano M, Shiba H, Miwa T, Che FS, Takayama S, Nagai T, Miyawaki A, Isogai A. Ca2+ dynamics in a pollen grain and papilla cell during pollination of Arabidopsis. Plant Physiology. 136: 3562-71. PMID 15489279 DOI: 10.1104/pp.104.046961  0.536
2004 Okamoto K, Nagai T, Miyawaki A, Hayashi Y. Rapid and persistent modulation of actin dynamics regulates postsynaptic reorganization underlying bidirectional plasticity. Nature Neuroscience. 7: 1104-12. PMID 15361876 DOI: 10.1038/nn1311  0.42
2004 Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proceedings of the National Academy of Sciences of the United States of America. 101: 10554-9. PMID 15247428 DOI: 10.1073/Pnas.0400417101  0.617
2004 Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W. Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control. Plos Biology. 2: e163. PMID 15208716 DOI: 10.1371/Journal.Pbio.0020163  0.646
2004 Nagai T, Miyawaki A. A high-throughput method for development of FRET-based indicators for proteolysis. Biochemical and Biophysical Research Communications. 319: 72-7. PMID 15158444 DOI: 10.1016/j.bbrc.2004.04.147  0.562
2004 Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. The Biochemical Journal. 381: 307-12. PMID 15065984 DOI: 10.1042/BJ20040321  0.668
2004 Miyawaki A, Nagai T, Mizuno H. Mechanisms of protein fluorophore formation and engineering. Current Opinion in Chemical Biology. 7: 557-62. PMID 14580558 DOI: 10.1016/s1367-5931(03)00097-8  0.676
2003 Miyawaki A, Mizuno H, Nagai T, Sawano A. Development of genetically encoded fluorescent indicators for calcium. Methods in Enzymology. 360: 202-25. PMID 12622151 DOI: 10.1016/s0076-6879(03)60111-4  0.655
2003 Takemoto K, Nagai T, Miyawaki A, Miura M. Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects. The Journal of Cell Biology. 160: 235-43. PMID 12527749 DOI: 10.1083/jcb.200207111  0.488
2002 Rekas A, Alattia JR, Nagai T, Miyawaki A, Ikura M. Crystal structure of venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity. The Journal of Biological Chemistry. 277: 50573-8. PMID 12370172 DOI: 10.1074/jbc.M209524200  0.521
2002 Shimozono S, Fukano T, Nagai T, Kirino Y, Mizuno H, Miyawaki A. Confocal imaging of subcellular Ca2+ concentrations using a dual-excitation ratiometric indicator based on green fluorescent protein. Science's Stke : Signal Transduction Knowledge Environment. 2002: pl4. PMID 11917155 DOI: 10.1126/Stke.2002.125.Pl4  0.701
2002 Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nature Biotechnology. 20: 87-90. PMID 11753368 DOI: 10.1038/nbt0102-87  0.647
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