1985 |
Burry, Richard W |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Effects of Antimitotic Drugs On Cns Regeneration
The object of the proposed research is to investigate the possibility that reactive non-neuronal cells at the site of a lesion can phagocytose growing axons and their growth cones as they attempt to cross the lesion. The model system used will be the formation and maintenance of apparent presynaptic elements on polylysine coated beads. The apparent presynaptic elements have been shown in cultures of the cerebellum to be neuronal swellings with 40 nm vesicles and with a density acumulated at the membrane in contact with the bead. The apparent presynaptic elements appear to be removed from the beads by non-neuronal cells, but preliminary results show this effect is inhibited by antimitotic drugs which stop non-neuronal cell division. The proposed research will examine the effect of antimitotic drugs on survival of apparent presynaptic elements both in vitro and in vivo. The first studies (I) are in vitro and will examine the effect of different antimitotic drugs on survival of apparent presynaptic elements. In this section additional experiments will use immunocytochemistry to identify the non-neuronal cell types that grow up onto the beads. The second studies (II) are in vivo and will examine the effect of antimitotic drugs on the formation of apparent presynaptic elements in 3-4 day old rats. Preliminary results show that neurons in the cerebellum of 3-4 day old rats do form apparent presynaptic elements on implanted beads. The maintenance of these apparent presynaptic elements and the effect of antimitotic drugs on their survival will be evaluated. The last group (III) of experiments will examine the response of adult cerebellar neurons to polylysine coated beads and the effect of antimitotic drugs on apparent presynaptic element survival. The proposed studies offer a new approach to the understanding of regeneration at the site of lesion. Is the phagocytic response of some non-neuronal cells at the site of CNS lesion possibly involved in the inhibition of axonal regeneration?
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1986 — 1987 |
Bishop, Georgia (co-PI) [⬀] King, James (co-PI) [⬀] Burry, Richard Ho, Raymond Beattie, Michael (co-PI) [⬀] |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Acquisition of a Cryo-Ultramicrotome |
0.915 |
1988 |
Burry, Richard W |
S10Activity Code Description: To make available to institutions with a high concentration of NIH extramural research awards, research instruments which will be used on a shared basis. |
Multi-User Application of An Electron Microscope
The Ohio State University has established the Central Electron Optics Facility (CEOF) to serve the entire campus with state-of- the-art instrumentation. This request for a scanning-transmission electron microscope (STEM) is made by a diverse group of users in the Bio-Medical Branch of the CEOF. With the extreme age of the existing electron microscopes and the demands for an instrument with better capabilities, the purchase of a new STEM will allow the major user group the new facilities to carry out their research. With a strong commitment from the University as well as the College of Medicine, the requested instrument will be installed, maintained and serviced. The major user group consists of 6 scientists, with five having current NIH support.
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2003 |
Burry, Richard W |
S10Activity Code Description: To make available to institutions with a high concentration of NIH extramural research awards, research instruments which will be used on a shared basis. |
Scanning Laser Confocal Microscope
DESCRIPTION (provided by applicant): The Campus Microscopy and Imaging Facility (CMIF) is requesting a Zeiss LSM 510 META. The CMIF is the core biomedical microscopy facility at the Ohio State University (OSU). The requested instrument is a basic Zeiss 510 LSM with visible lasers (no UV laser), META detector, two PMTs, five objectives on an inverted stand, and a workstation. This instrument is needed because there is no state-of-the-art confocal microscope available to the biomedical research faculty and students at OSU. Currently, the CMIF has an aging BioRad MRC 600, which is technically limited, unreliable and inadequate for the studies proposed here. On the OSU campus there are four confocals microscopes, a BioRad 1024, a Nikon PCM-2000, a Zeiss 410 and a Zeiss 510 NLO. All of these are in individual faculty labs, or only available to extremely restricted groups of faculty and certainly not to the NIH supported research community. Seven major users, with NIH funding, and four minor users with NSF or private foundation funding, are submitting this application. The NIH projects that require confocal microscopy are investigating axon sprouting in spinal cord regeneration, immune cell response to spinal cord injury, initiation of preterm labor and delivery, causes of amyotrophic lateral sclerosis, IgG transport across the placenta, CIRF role as a neurotrophin in cerebellar development, and the role for serotonin in stimulating supraspinal pain. The University will provide continuing institutional support in the form of staff salary and space allocation for this instrument. Acquisition of the Zeiss 510 LSM META will significantly enhance the research programs of the major users and aid other investigators in the university community.
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2009 |
Burry, Richard W |
S10Activity Code Description: To make available to institutions with a high concentration of NIH extramural research awards, research instruments which will be used on a shared basis. |
Scanning Laser Confocal For Core Facility
DESCRIPTION (provided by applicant): Campus Microscopy and Imaging Facility (CMIF) is the core biomedical microscope facility on the campus of the Ohio State University. The CMIF obtained a Zeiss 510 META on an upright microscope with a S10 grant in 2004 and it has served as a workhorse for four years. The Zeiss is currently used more than 2400 hours per year and is reserved for at least a week in advance. The CMIF has just moved into the new ten storeys Biomedical Research Tower which will house 188 research scientists. While the Zeiss 510 continues to serve the CMIF, a second routine confocal microscope is needed to handle the increased usage. In this application six NIH funded investigators show how the Zeiss 510 has given them crucial data for their research. The instrument requested scanning laser system will be on an upright stand, with high numerical oil objectives, 405 nm diode laser, Argon laser, green HeNe, Red HeNe, three PMTs (no spectral detectors) and easy to use software. Demos on the Zeiss 510 and FV1000 showed these instruments to fit our needs. Because of the changes in this market at the time of submission (March 2008), we are requesting funds to purchase either a Zeiss or Olympus microscope and will make the decision as to manufacturer and model in a year, if the application is funded. PUBLIC HEALTH RELEVANCE: The microscope requested in this application will allow currently funded NIH researchers to perform their critical research. Some of the health issues addressed in this research include: neuromuscular disease, spinal cord injury, cancer, lung disease, and bone reabsorption disease.
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2012 — 2015 |
Burry, Richard W |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Microscopy
PROJECT SUMMARY (See instructions): The Microscopy Shared Resource (MSR) is a centrally-located resource with cutting-edge instruments and highly trained experts to provide outstanding support for OSUCCC scientists for confocal, light, scanning electron and transmission electron microscopy, the MSR has two electron microscopes and four confocals all purchased since 2005 through federal grants and outstanding institutional support. These instruments include two single photon Olympus FVI 000 confocal microscopes each with four lasers and high N.A. objectives specifically for fixed cells and tissue, an FEI Tecnai BioTwin transmission electron microscope, and an Olympus FVI 000 multiphoton confocal instrument with a MaiTai DeepSee laser to probe deep into tumors in both live animals and fixed tissue. The MSR is led by Dr. Richard Burry, an established and well funded scientist with over 30 years of extensive expertise in microscopy, who along with an experienced and highly-trained staff, provides OSUCCC investigators vital consultation in experimental design and image analysis. Usage and productivity from the MSR is enhanced by well-organized training courses and individual training offered by staff members. The MSR is extensively used across OSUCCC scientific programs providing service to >30 OSUCCC member labs to generate the images for high quality cancer relevant publications and grants. The MSR is centrally located on the second floor of the Biomedical Research Tower (BRT) close to the labs of the OSUCCC members. Based on the expanding capabilities of the MSR and increases in number of grant applications from OSUCCC members, the OSUCCC usage is estimated to increase dramatically over the next five years based on strategic recruitment goals and expanded demand for high-end microscopy in cancer research. The MSR is supported by outstanding institutional resources by leveraging extensive partnerships with OSU Colleges, the OSU Office of Research, grants from the State of Ohio and the OSUCCC. The MSR is a new OSUCCC shared resource, previously with a strong user base as an OSU core facility, and thus fulfills NIH goals to consolidate core facilities for maximal efficiency and utilization by NIH funded investigators. Collectively the MSR is a critical shared resource for OSUCCC investigators seeking to identify specific cells and proteins in normal tissue and in tumors to enhance our understanding of fundamental processes of cancer in developing therapeutic strategies.
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