René F. Ketting, PhD - Publications

Affiliations: 
RNA silencing Hubrecht Institute 
Area:
RNA interference
Website:
http://hubrecht.eu/research/ketting/index.html

79 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2024 Haase AD, Ketting RF, Lai EC, van Rij RP, Siomi M, Svoboda P, van Wolfswinkel JC, Wu PH. PIWI-interacting RNAs: who, what, when, where, why, and how. The Embo Journal. PMID 39327528 DOI: 10.1038/s44318-024-00253-8  0.422
2024 Busetto V, Pshanichnaya L, Lichtenberger R, Hann S, Ketting RF, Falk S. MUT-7 exoribonuclease activity and localization are mediated by an ancient domain. Nucleic Acids Research. 52: 9076-9091. PMID 39188014 DOI: 10.1093/nar/gkae610  0.321
2023 Bronkhorst AW, Lee CY, Möckel MM, Ruegenberg S, de Jesus Domingues AM, Sadouki S, Piccinno R, Sumiyoshi T, Siomi MC, Stelzl L, Luck K, Ketting RF. An extended Tudor domain within Vreteno interconnects Gtsf1L and Ago3 for piRNA biogenesis in Bombyx mori. The Embo Journal. e114072. PMID 37984437 DOI: 10.15252/embj.2023114072  0.358
2023 Podvalnaya N, Bronkhorst AW, Lichtenberger R, Hellmann S, Nischwitz E, Falk T, Karaulanov E, Butter F, Falk S, Ketting RF. piRNA processing by a trimeric Schlafen-domain nuclease. Nature. 622: 402-409. PMID 37758951 DOI: 10.1038/s41586-023-06588-2  0.451
2022 Marnik EA, Almeida MV, Cipriani PG, Chung G, Caspani E, Karaulanov E, Gan HH, Zinno J, Isolehto IJ, Kielisch F, Butter F, Sharp CS, Flanagan RM, Bonnet FX, Piano F, ... Ketting RF, et al. The Caenorhabditis elegans TDRD5/7-like protein, LOTR-1, interacts with the helicase ZNFX-1 to balance epigenetic signals in the germline. Plos Genetics. 18: e1010245. PMID 35657999 DOI: 10.1371/journal.pgen.1010245  0.391
2022 Schreier J, Dietz S, Boermel M, Oorschot V, Seistrup AS, de Jesus Domingues AM, Bronkhorst AW, Nguyen DAH, Phillis S, Gleason EJ, L'Hernault SW, Phillips CM, Butter F, Ketting RF. Membrane-associated cytoplasmic granules carrying the Argonaute protein WAGO-3 enable paternal epigenetic inheritance in Caenorhabditis elegans. Nature Cell Biology. 24: 217-229. PMID 35132225 DOI: 10.1038/s41556-021-00827-2  0.363
2021 Perez-Borrajero C, Podvalnaya N, Holleis K, Lichtenberger R, Karaulanov E, Simon B, Basquin J, Hennig J, Ketting RF, Falk S. Structural basis of PETISCO complex assembly during piRNA biogenesis in . Genes & Development. PMID 34413138 DOI: 10.1101/gad.348648.121  0.429
2021 Ketting RF, Cochella L. Concepts and functions of small RNA pathways in C. elegans. Current Topics in Developmental Biology. 144: 45-89. PMID 33992161 DOI: 10.1016/bs.ctdb.2020.08.002  0.465
2021 Gudipati RK, Braun K, Gypas F, Hess D, Schreier J, Carl SH, Ketting RF, Großhans H. Protease-mediated processing of Argonaute proteins controls small RNA association. Molecular Cell. PMID 33852894 DOI: 10.1016/j.molcel.2021.03.029  0.464
2020 Redl S, de Jesus Domingues AM, Caspani E, Möckel S, Salvenmoser W, Mendez-Lago M, Ketting RF. Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish. Development (Cambridge, England). PMID 33298460 DOI: 10.1242/dev.193060  0.314
2020 Placentino M, de Jesus Domingues AM, Schreier J, Dietz S, Hellmann S, de Albuquerque BF, Butter F, Ketting RF. Intrinsically disordered protein PID-2 modulates Z granules and is required for heritable piRNA-induced silencing in the Caenorhabditis elegans embryo. The Embo Journal. e105280. PMID 33231880 DOI: 10.15252/embj.2020105280  0.518
2020 Manage KI, Rogers AK, Wallis DC, Uebel CJ, Anderson DC, Nguyen DAH, Arca K, Brown KC, Cordeiro Rodrigues RJ, de Albuquerque BFM, Ketting RF, Montgomery TA, Phillips CM. A Tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in . Elife. 9. PMID 32338603 DOI: 10.7554/Elife.56731  0.474
2020 Manage KI, Rogers AK, Wallis DC, Uebel CJ, Anderson DC, Nguyen DAH, Arca K, Brown KC, Rodrigues RJC, Albuquerque BFd, Ketting RF, Montgomery TA, Phillips CM. Author response: A tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in C. elegans Elife. DOI: 10.7554/Elife.56731.Sa2  0.347
2019 Cordeiro Rodrigues RJ, de Jesus Domingues AM, Hellmann S, Dietz S, de Albuquerque BFM, Renz C, Ulrich HD, Sarkies P, Butter F, Ketting RF. PETISCO is a novel protein complex required for 21U RNA biogenesis and embryonic viability. Genes & Development. PMID 31147388 DOI: 10.1101/Gad.322446.118  0.584
2019 Almeida MV, de Jesus Domingues AM, Lukas H, Mendez-Lago M, Ketting RF. RppH can faithfully replace TAP to allow cloning of 5'-triphosphate carrying small RNAs. Methodsx. 6: 265-272. PMID 30788220 DOI: 10.1016/J.Mex.2019.01.009  0.551
2019 Almeida MV, de Jesus Domingues AM, Ketting RF. Maternal and zygotic gene regulatory effects of endogenous RNAi pathways. Plos Genetics. 15: e1007784. PMID 30759082 DOI: 10.1371/Journal.Pgen.1007784  0.543
2019 Almeida MV, Andrade-Navarro MA, Ketting RF. Function and Evolution of Nematode RNAi Pathways. Non-Coding Rna. 5. PMID 30650636 DOI: 10.3390/Ncrna5010008  0.502
2018 Almeida MV, Dietz S, Redl S, Karaulanov E, Hildebrandt A, Renz C, Ulrich HD, König J, Butter F, Ketting RF. GTSF-1 is required for formation of a functional RNA-dependent RNA Polymerase complex in . The Embo Journal. PMID 29769402 DOI: 10.15252/Embj.201899325  0.578
2018 Bronkhorst AW, Ketting RF. Trimming it short: PNLDC1 is required for piRNA maturation during mouse spermatogenesis. Embo Reports. PMID 29459487 DOI: 10.15252/Embr.201845824  0.314
2016 Lebedeva S, de Jesus Domingues AM, Butter F, Ketting RF. Characterization of genetic loss-of-function of Fus in zebrafish. Rna Biology. 0. PMID 27898262 DOI: 10.1080/15476286.2016.1256532  0.385
2016 San B, Chrispijn ND, Wittkopp N, van Heeringen SJ, Lagendijk AK, Aben M, Bakkers J, Ketting RF, Kamminga LM. Normal formation of a vertebrate body plan and loss of tissue maintenance in the absence of ezh2. Scientific Reports. 6: 24658. PMID 27145952 DOI: 10.1038/Srep24658  0.35
2016 Kloosterman WP, Wienholds E, Ketting RF, Plasterk RH. Substrate requirements for let-7 function in the developing zebrafish embryo. Nucleic Acids Research. PMID 26969732 DOI: 10.1093/nar/gkw173  0.512
2015 Rosenkranz D, Han CT, Roovers EF, Zischler H, Ketting RF. Piwi proteins and piRNAs in mammalian oocytes and early embryos: From sample to sequence. Genomics Data. 5: 309-13. PMID 26484274 DOI: 10.1016/J.Gdata.2015.06.026  0.408
2015 de Albuquerque BF, Placentino M, Ketting RF. Maternal piRNAs Are Essential for Germline Development following De Novo Establishment of Endo-siRNAs in Caenorhabditis elegans. Developmental Cell. 34: 448-56. PMID 26279485 DOI: 10.1016/J.Devcel.2015.07.010  0.514
2015 Gebert D, Ketting RF, Zischler H, Rosenkranz D. piRNAs from Pig Testis Provide Evidence for a Conserved Role of the Piwi Pathway in Post-Transcriptional Gene Regulation in Mammals. Plos One. 10: e0124860. PMID 25950437 DOI: 10.1371/Journal.Pone.0124860  0.437
2015 Roovers EF, Rosenkranz D, Mahdipour M, Han CT, He N, Chuva de Sousa Lopes SM, van der Westerlaken LA, Zischler H, Butter F, Roelen BA, Ketting RF. Piwi proteins and piRNAs in mammalian oocytes and early embryos. Cell Reports. 10: 2069-82. PMID 25818294 DOI: 10.1016/J.Celrep.2015.02.062  0.349
2015 Rosenkranz D, Rudloff S, Bastuck K, Ketting RF, Zischler H. Tupaia small RNAs provide insights into function and evolution of RNAi-based transposon defense in mammals. Rna (New York, N.Y.). 21: 911-22. PMID 25802409 DOI: 10.1261/Rna.048603.114  0.557
2014 Swarts DC, Makarova K, Wang Y, Nakanishi K, Ketting RF, Koonin EV, Patel DJ, van der Oost J. The evolutionary journey of Argonaute proteins. Nature Structural & Molecular Biology. 21: 743-53. PMID 25192263 DOI: 10.1038/Nsmb.2879  0.398
2014 de Albuquerque BF, Luteijn MJ, Cordeiro Rodrigues RJ, van Bergeijk P, Waaijers S, Kaaij LJ, Klein H, Boxem M, Ketting RF. PID-1 is a novel factor that operates during 21U-RNA biogenesis in Caenorhabditis elegans. Genes & Development. 28: 683-8. PMID 24696453 DOI: 10.1101/Gad.238220.114  0.563
2014 Phillips CM, Montgomery BE, Breen PC, Roovers EF, Rim YS, Ohsumi TK, Newman MA, van Wolfswinkel JC, Ketting RF, Ruvkun G, Montgomery TA. MUT-14 and SMUT-1 DEAD box RNA helicases have overlapping roles in germline RNAi and endogenous siRNA formation. Current Biology : Cb. 24: 839-44. PMID 24684932 DOI: 10.1016/J.Cub.2014.02.060  0.452
2014 Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Profiling of RNA modifications by multiplexed stable isotope labelling. Chemical Communications (Cambridge, England). 50: 3516-8. PMID 24567952 DOI: 10.1039/C3Cc49114E  0.421
2014 Huang HY, Ketting RF. Isolation of zebrafish gonads for RNA isolation. Methods in Molecular Biology (Clifton, N.J.). 1093: 183-94. PMID 24178566 DOI: 10.1007/978-1-62703-694-8_15  0.411
2013 de Albuquerque BF, Ketting RF. Is this mine? small RNAs help to decide. Developmental Cell. 27: 599-601. PMID 24369831 DOI: 10.1016/J.Devcel.2013.12.005  0.389
2013 Luteijn MJ, Ketting RF. PIWI-interacting RNAs: from generation to transgenerational epigenetics. Nature Reviews. Genetics. 14: 523-34. PMID 23797853 DOI: 10.1038/Nrg3495  0.564
2013 Kaaij LJ, Hoogstrate SW, Berezikov E, Ketting RF. piRNA dynamics in divergent zebrafish strains reveal long-lasting maternal influence on zygotic piRNA profiles. Rna (New York, N.Y.). 19: 345-56. PMID 23335638 DOI: 10.1261/Rna.036400.112  0.326
2012 Luteijn MJ, van Bergeijk P, Kaaij LJ, Almeida MV, Roovers EF, Berezikov E, Ketting RF. Extremely stable Piwi-induced gene silencing in Caenorhabditis elegans. The Embo Journal. 31: 3422-30. PMID 22850670 DOI: 10.1038/Emboj.2012.213  0.481
2012 Kamminga LM, van Wolfswinkel JC, Luteijn MJ, Kaaij LJ, Bagijn MP, Sapetschnig A, Miska EA, Berezikov E, Ketting RF. Differential impact of the HEN1 homolog HENN-1 on 21U and 26G RNAs in the germline of Caenorhabditis elegans. Plos Genetics. 8: e1002702. PMID 22829772 DOI: 10.1371/Journal.Pgen.1002702  0.57
2011 Ketting RF. microRNA Biogenesis and Function : An overview. Advances in Experimental Medicine and Biology. 700: 1-14. PMID 21755468 DOI: 10.1007/978-1-4419-7823-3_1  0.582
2011 Huang HY, Houwing S, Kaaij LJ, Meppelink A, Redl S, Gauci S, Vos H, Draper BW, Moens CB, Burgering BM, Ladurner P, Krijgsveld J, Berezikov E, Ketting RF. Tdrd1 acts as a molecular scaffold for Piwi proteins and piRNA targets in zebrafish. The Embo Journal. 30: 3298-308. PMID 21743441 DOI: 10.1038/Emboj.2011.228  0.774
2011 Kamminga LM, Ketting RF. RNAi genes pave their own way. Genes & Development. 25: 529-33. PMID 21406552 DOI: 10.1101/Gad.2038611  0.33
2011 Ketting RF. The many faces of RNAi. Developmental Cell. 20: 148-61. PMID 21316584 DOI: 10.1016/J.Devcel.2011.01.012  0.537
2010 Kamminga LM, Luteijn MJ, den Broeder MJ, Redl S, Kaaij LJ, Roovers EF, Ladurner P, Berezikov E, Ketting RF. Hen1 is required for oocyte development and piRNA stability in zebrafish. The Embo Journal. 29: 3688-700. PMID 20859253 DOI: 10.1038/Emboj.2010.233  0.554
2010 van Wolfswinkel JC, Ketting RF. The role of small non-coding RNAs in genome stability and chromatin organization. Journal of Cell Science. 123: 1825-39. PMID 20484663 DOI: 10.1242/Jcs.061713  0.552
2010 Corrêa RL, Steiner FA, Berezikov E, Ketting RF. MicroRNA-directed siRNA biogenesis in Caenorhabditis elegans. Plos Genetics. 6: e1000903. PMID 20386745 DOI: 10.1371/Journal.Pgen.1000903  0.521
2009 den Broeder MJ, van der Linde H, Brouwer JR, Oostra BA, Willemsen R, Ketting RF. Generation and characterization of FMR1 knockout zebrafish. Plos One. 4: e7910. PMID 19936290 DOI: 10.1371/Journal.Pone.0007910  0.303
2009 van Wolfswinkel JC, Claycomb JM, Batista PJ, Mello CC, Berezikov E, Ketting RF. CDE-1 affects chromosome segregation through uridylation of CSR-1-bound siRNAs. Cell. 139: 135-48. PMID 19804759 DOI: 10.1016/J.Cell.2009.09.012  0.356
2009 Claycomb JM, Batista PJ, Pang KM, Gu W, Vasale JJ, van Wolfswinkel JC, Chaves DA, Shirayama M, Mitani S, Ketting RF, Conte D, Mello CC. The Argonaute CSR-1 and its 22G-RNA cofactors are required for holocentric chromosome segregation. Cell. 139: 123-34. PMID 19804758 DOI: 10.1016/J.Cell.2009.09.014  0.51
2009 Steiner FA, Okihara KL, Hoogstrate SW, Sijen T, Ketting RF. RDE-1 slicer activity is required only for passenger-strand cleavage during RNAi in Caenorhabditis elegans. Nature Structural & Molecular Biology. 16: 207-11. PMID 19151723 DOI: 10.1038/Nsmb.1541  0.58
2008 Houwing S, Berezikov E, Ketting RF. Zili is required for germ cell differentiation and meiosis in zebrafish. The Embo Journal. 27: 2702-11. PMID 18833190 DOI: 10.1038/Emboj.2008.204  0.809
2008 Das PP, Bagijn MP, Goldstein LD, Woolford JR, Lehrbach NJ, Sapetschnig A, Buhecha HR, Gilchrist MJ, Howe KL, Stark R, Matthews N, Berezikov E, Ketting RF, Tavaré S, Miska EA. Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline. Molecular Cell. 31: 79-90. PMID 18571451 DOI: 10.1016/J.Molcel.2008.06.003  0.589
2008 Ketting R, Houwing S, Kamminga L, Huang H. Small RNAs in animal development European Journal of Cancer Supplements. 6: 121. DOI: 10.1016/S1359-6349(08)71637-9  0.783
2007 Ketting RF. A dead end for microRNAs. Cell. 131: 1226-7. PMID 18160032 DOI: 10.1016/J.Cell.2007.12.004  0.464
2007 Steiner FA, Hoogstrate SW, Okihara KL, Thijssen KL, Ketting RF, Plasterk RH, Sijen T. Structural features of small RNA precursors determine Argonaute loading in Caenorhabditis elegans. Nature Structural & Molecular Biology. 14: 927-33. PMID 17891148 DOI: 10.1038/Nsmb1308  0.73
2007 Kloosterman WP, Lagendijk AK, Ketting RF, Moulton JD, Plasterk RH. Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development. Plos Biology. 5: e203. PMID 17676975 DOI: 10.1371/Journal.Pbio.0050203  0.573
2007 Houwing S, Kamminga LM, Berezikov E, Cronembold D, Girard A, van den Elst H, Filippov DV, Blaser H, Raz E, Moens CB, Plasterk RH, Hannon GJ, Draper BW, Ketting RF. A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish. Cell. 129: 69-82. PMID 17418787 DOI: 10.1016/J.Cell.2007.03.026  0.765
2006 Ketting RF, Tijsterman M, Plasterk RH. Isolation of RNA from C. elegans. Csh Protocols. 2006. PMID 22485697 DOI: 10.1101/Pdb.Prot4321  0.697
2006 Ketting RF, Tijsterman M, Plasterk RH. Detection of si/miRNA in C. elegans by Northern Blot. Csh Protocols. 2006. PMID 22485696 DOI: 10.1101/Pdb.Prot4320  0.567
2006 Ketting RF, Tijsterman M, Plasterk RH. Detection of siRNA in C. elegans Using RNase Protection. Csh Protocols. 2006. PMID 22485695 DOI: 10.1101/Pdb.Prot4319  0.588
2006 Ketting RF, Tijsterman M, Plasterk RH. Cosuppression in C. elegans. Csh Protocols. 2006. PMID 22485694 DOI: 10.1101/Pdb.Prot4318  0.552
2006 Ketting RF, Tijsterman M, Plasterk RH. Introduction of Double-Stranded RNA in C. elegans by Feeding. Csh Protocols. 2006. PMID 22485693 DOI: 10.1101/pdb.prot4317  0.686
2006 Ketting RF, Tijsterman M, Plasterk RH. Introduction of Double-Stranded RNA in C. elegans by Soaking. Csh Protocols. 2006. PMID 22485692 DOI: 10.1101/Pdb.Prot4316  0.713
2006 Ketting RF, Tijsterman M, Plasterk RH. Introduction of Double-Stranded RNA in C. elegans by Injection. Csh Protocols. 2006. PMID 22485691 DOI: 10.1101/Pdb.Prot4315  0.704
2006 Tops BB, Plasterk RH, Ketting RF. The Caenorhabditis elegans Argonautes ALG-1 and ALG-2: almost identical yet different. Cold Spring Harbor Symposia On Quantitative Biology. 71: 189-94. PMID 17381296 DOI: 10.1101/sqb.2006.71.035  0.705
2006 Ketting RF. Partners in dicing. Genome Biology. 7: 210. PMID 16584538 DOI: 10.1186/Gb-2006-7-3-210  0.513
2005 Tops BB, Tabara H, Sijen T, Simmer F, Mello CC, Plasterk RH, Ketting RF. RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans. Nucleic Acids Research. 33: 347-55. PMID 15653635 DOI: 10.1093/Nar/Gki183  0.738
2004 Kloosterman WP, Wienholds E, Ketting RF, Plasterk RH. Substrate requirements for let-7 function in the developing zebrafish embryo. Nucleic Acids Research. 32: 6284-91. PMID 15585662 DOI: 10.1093/Nar/Gkh968  0.618
2004 Denli AM, Tops BB, Plasterk RH, Ketting RF, Hannon GJ. Processing of primary microRNAs by the Microprocessor complex. Nature. 432: 231-5. PMID 15531879 DOI: 10.1038/Nature03049  0.629
2004 Ketting RF, Plasterk RH. What's new about RNAi? Meeting on siRNAs and miRNAs. Embo Reports. 5: 762-5. PMID 15272300 DOI: 10.1038/Sj.Embor.7400207  0.669
2003 Caudy AA, Ketting RF, Hammond SM, Denli AM, Bathoorn AM, Tops BB, Silva JM, Myers MM, Hannon GJ, Plasterk RH. A micrococcal nuclease homologue in RNAi effector complexes. Nature. 425: 411-4. PMID 14508492 DOI: 10.1038/Nature01956  0.726
2003 Krijgsveld J, Ketting RF, Mahmoudi T, Johansen J, Artal-Sanz M, Verrijzer CP, Plasterk RH, Heck AJ. Metabolic labeling of C. elegans and D. melanogaster for quantitative proteomics. Nature Biotechnology. 21: 927-31. PMID 12858183 DOI: 10.1038/Nbt848  0.564
2002 Tijsterman M, Ketting RF, Plasterk RH. The genetics of RNA silencing. Annual Review of Genetics. 36: 489-519. PMID 12429701 DOI: 10.1146/Annurev.Genet.36.043002.091619  0.721
2002 Tijsterman M, Ketting RF, Okihara KL, Sijen T, Plasterk RH. RNA helicase MUT-14-dependent gene silencing triggered in C. elegans by short antisense RNAs. Science (New York, N.Y.). 295: 694-7. PMID 11809977 DOI: 10.1126/Science.1067534  0.725
2001 Ketting RF, Fischer SE, Bernstein E, Sijen T, Hannon GJ, Plasterk RH. Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes & Development. 15: 2654-9. PMID 11641272 DOI: 10.1101/Gad.927801  0.753
2000 Plasterk RH, Ketting RF. The silence of the genes. Current Opinion in Genetics & Development. 10: 562-7. PMID 10980436 DOI: 10.1016/S0959-437X(00)00128-3  0.683
2000 Ketting RF, Plasterk RH. A genetic link between co-suppression and RNA interference in C. elegans. Nature. 404: 296-8. PMID 10749214 DOI: 10.1038/35005113  0.718
1999 Ketting RF, Haverkamp TH, van Luenen HG, Plasterk RH. Mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD. Cell. 99: 133-41. PMID 10535732 DOI: 10.1016/S0092-8674(00)81645-1  0.668
1997 Ketting RF, Fischer SE, Plasterk RH. Target choice determinants of the Tc1 transposon of Caenorhabditis elegans. Nucleic Acids Research. 25: 4041-7. PMID 9321655 DOI: 10.1093/nar/25.20.4041  0.511
1997 van Pouderoyen G, Ketting RF, Perrakis A, Plasterk RH, Sixma TK. Crystal structure of the specific DNA-binding domain of Tc3 transposase of C.elegans in complex with transposon DNA. The Embo Journal. 16: 6044-54. PMID 9312061 DOI: 10.1093/Emboj/16.19.6044  0.507
1996 Bang DD, Ketting R, de Ruijter M, Brandsma JA, Verhage RA, van de Putte P, Brouwer J. Cloning of Schizosaccharomyces pombe rph16+, a gene homologous to the Saccharomyces cerevisiae RAD16 gene. Mutation Research. 364: 57-71. PMID 8879272 DOI: 10.1016/0921-8777(96)00010-9  0.518
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